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Institut de biologie
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+41 32 718 30 00
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+41 32 718 30 01
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Rue Emile-Argand 11 (bureaux D013 et D016 - bât. D - rez-de-chaussée)
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2000
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Neuchâtel
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CH
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Academic Institute
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Voici les éléments 1 - 10 sur 2544
- PublicationMétadonnées seulementRegeneration of a lytic central vacuole and of neutral peripheral vacuoles can be visualized by green fluorescent proteins targeted to either type of vacuoles(2001)
; Protein trafficking to two different types of vacuoles was investigated in tobacco (Nicotiana tabacum cv SR1) mesophyll protoplasts using two different vacuolar green fluorescent proteins (GFPs). One GFP is targeted to a pH-neutral vacuole by the C-terminal vacuolar sorting determinant of tobacco chitinase A, whereas the other GFP is targeted to an acidic lytic vacuole by the N-terminal propeptide of barley aleurain, which contains a sequence-specific vacuolar sorting determinant. The trafficking and final accumulation in the central vacuole (CV) or in smaller peripheral vacuoles differed for the two reporter proteins, depending on the cell type. Within 2 d, evacuolated (mini-) protoplasts regenerate a large CV. Expression of the two vacuolar GFPs in miniprotoplasts indicated that the newly formed CV was a lytic vacuole, whereas neutral vacuoles always remained peripheral. Only later, once the regeneration of the CV was completed, the content of peripheral storage vacuoles could be seen to appear in the CV of a third of the cells, apparently by heterotypic fusion. - PublicationMétadonnées seulementCross-reactive acquired immunity influences transmission success of the Lyme disease pathogen, Borrelia afzelii(2015-12)
; ; ; Cross-reactive acquired immunity in the vertebrate host induces indirect competition between strains of a given pathogen species and is critical for understanding the ecology of mixed infections. In vector-borne diseases, cross-reactive antibodies can reduce pathogen transmission at the vector-to-host and the host-to-vector lifecycle transition. The highly polymorphic, immunodominant, outer surface protein C (OspC) of the tick-borne spirochete bacterium Borrelia afzelii induces a strong antibody response in the vertebrate host. To test how cross-immunity in the vertebrate host influences tick-to-host and host-to-tick transmission, mice were immunized with one of two strain-specific recombinant OspC proteins (A3, A10), challenged via tick bite with one of the two B. afzelii ospC strains (A3, A10), and infested with xenodiagnostic ticks. Immunization with a given rOspC antigen protected mice against homologous strains carrying the same major ospC group allele but provided little or no cross-protection against heterologous strains carrying a different major ospC group allele. There were cross-immunity effects on the tick spirochete load but not on the probability of host-to-tick transmission. The spirochete load in ticks that had fed on mice with cross-immune experience was reduced by a factor of two compared to ticks that had fed on naive control mice. In addition, strain-specific differences in mouse spirochete load, host-to-tick transmission, tick spirochete load, and the OspC-specific IgG response revealed the mechanisms that determine variation in transmission success between strains of B. afzelii. This study shows that cross-immunity in infected vertebrate hosts can reduce pathogen load in the arthropod vector with potential consequences for vector-to-host pathogen transmission. (C) 2015 Elsevier B.V. All rights reserved. - PublicationMétadonnées seulementAcquired resistance in Arabidopsis thaliana(1992)
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- PublicationMétadonnées seulementSelection of Grifola frondosa and Fomitopsis pinicola strains resistant to Trichoderma viride in teflon tubes confrontation method(2004)
; Hmamda, Abdelaziz - PublicationMétadonnées seulementProtozoan and bacterial pathogens in tick salivary glands in wild and domestic animal environments in South Africa(2014)
- PublicationMétadonnées seulementAdult Cleaner Wrasse Outperform Capuchin Monkeys, Chimpanzees and Orang-utans in a Complex Foraging Task Derived from Cleaner - Client Reef Fish Cooperation(2012)
; - PublicationMétadonnées seulementAntennal electrophysiological responses of three parasitic wasps to caterpillar-induced volatiles from maize (Zea mays mays), cotton (Gossypium herbaceum), and cowpea (Vigna unguiculata)(2005)
; - PublicationMétadonnées seulementA global analysis of elevational gradients in leaf herbivory and its underlying drivers: effects of plant growth form, leaf habit, and climatic correlates(2017-9-16)
; Moreira, Xoaquin