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Turlings, Ted

Nom
Turlings, Ted
Affiliation principale
Fonction
Professeur ordinaire
Email
ted.turlings@unine.ch
Identifiants
https://libra.unine.ch/handle/123456789/330

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  • Publication
    Accès libre
    Parasotoid communities and genetic structure: host plant does not matter
    (2008)
    Jourdie, Violaine
    ;
    Turlings, Ted 
    Plant-insect interactions have long been studied and reveal intricate mechanisms. Plants are capable of defending themselves both directly by poisoning insect herbivores and indirectly by emitting volatile compounds that are used by the natural enemies to localize their host. In response, insects have evolved strategies to defeat plant defense mechanisms. Because insect pests are affected by plant signals, their natural enemies also bear these effects. As host plant can affect the physiology and behavior of parasitoids, it may also contribute to shaping their population genetic structure. This thesis mainly aimed to investigate the effects of host plant on the population genetics of parasitoids of the fall armyworm (FAW), Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae), using microsatellite markers. The FAW is one of the New World’s most devastating pests and it attacks several economically important crops as well as grasses. It is commonly controlled by chemical insecticides. However, as it is attacked by numerous parasitoids species, and in order to limit the use of toxic pesticides, biological control is a safer alternative mean of management for this pest. The success of biological control relies on a good knowledge of the system, hence the importance of investigating population genetics and communities structure. This study focused on two primary parasitoids of S. frugiperda, Chelonus insularis Cresson (Hymenotera: Braconidae) and Campoletis sonorensis Cameron (Hymenoptera: Ichneumonidae). It was conducted on two host plants, maize and sorghum, in Mexico where maize originated and where sorghum was introduced barely over a century ago. Due to difficulties encountered during sampling, whereby immature parasitoids did not complete their life cycle and therefore could not be morphologically identified, a technique was first developed, as a cheaper and faster alternative to sequencing, to molecularly assign parasitoid larvae to species. This simple but nonetheless efficient technique consists in amplifying DNA through polymerase chain reaction and digesting it with a cocktail of restriction endonucleases in order to obtain a species specific pattern when the digestion product is run on an agarose gel. With this technique, we could get an accurate estimation of which species were collected and in what proportions, which allowed to study parasitoid community structure. The study of population genetics first required the development and optimization of reliable molecular markers. Fifteen and 13 highly polymorphic microsatellites were respectively isolated from C. sonorensis and from C. insularis. These markers were used to investigate fine-scale genetic structure in Mexican populations. We could discern a regional effect, but host plant seems to play no role in shaping the populations genetic structure. High levels of admixture indicate that gene flow between populations is considerable. Finally, genetic structure was investigated at larger scale through a phylogeography using sequences of mitochondrial and nuclear marker genes. The lack of local structure was confirmed for both species. We found however evidence for North-South migration through a single colonization event in C. insularis, and a cryptic species distributed in Canada was discovered. Dispersal of these insects seems to be largely driven by wind as suggested by genetic similarities between geographically very distant individuals.
  • Publication
    Accès libre
    Occurrence and direct control potential of parasitoids and predators of the fall armyworm (Lepidoptera: Noctuidae) on maize in the subtropical lowlands of Mexico
    (2004)
    Hoballah, Maria Elena
    ;
    Degen, Thomas 
    ;
    Bergvinson, David
    ;
    Savidan, Anita
    ;
    Tamò, Cristina
    ;
    Turlings, Ted 
    1 Native natural enemies have the potential to control fall armyworm Spodoptera frugiperda (Smith) in tropical maize grown in Mexico, where this insect pest causes severe economic losses to farmers. It has been proposed that enhancing herbivore-induced volatile emissions in maize plants may help to increase the effectiveness of natural enemies, which use these volatiles to locate their prey. This will only be of immediate benefit to farmers if the activity of the natural enemies results in a direct reduction in herbivory. Here we report on field surveys for the most common natural enemies in a tropical maize-growing region in Mexico and the potential effects of these enemies on herbivory by fall armyworm.
    2 Caterpillars were collected in maize fields near Poza Rica in the state of Veracruz during January and February 1999, 2000 and 2001. Plants were either naturally infested by S. frugiperda, or artificially infested with laboratory-reared larvae. Ten species of parasitoids emerged from the collected larvae and eight species of predators that are known to feed on larvae and eggs were observed on the plants. Campoletis sonorensis (Cameron) (Hymenoptera: Ichneumonidae) was the dominant parasitoid species, in 1999 and 2001.
    3 Of the nine larval parasitoids collected, six (all solitary) are known to reduce herbivory, whereas one causes the host to eat more (for two species this is not known). This implies that enhancing the effectiveness of solitary endoparasitoids may benefit subsistence farmers in developing countries by immediately reducing herbivory. The overall benefit for the plant resulting from parasitoid activity also has important implications for the evolutionary role of parasitoids in contributing to selection pressures that shape indirect defences in plants.