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Brossard, Michel
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Brossard, Michel
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Voici les éléments 1 - 10 sur 31
- PublicationAccès libreRole of soil macrofauna in P cycling(NY, USA: Springer, 2011)
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- PublicationMétadonnées seulementDoes access to the bluestreak cleaner wrasse Labroides dimidiatus affect indicators of stress and health in resident reef fishes in the Red Sea?(2011)
; ; - PublicationMétadonnées seulementEstimated Lyme borreliosis incidence in the canton of Neuchâtel (Switzerland)(2012-1-1)
; ; - PublicationAccès libreRole of Soil Macrofauna in Phosphorus Cycling(2011)
; ; - PublicationAccès libreIdentification of an Ixodes ricinus salivary gland fraction through its ability to stimulate CD4 T cells present in BALB/c mice lymph nodes draining the tick fixation site(1997)
- PublicationAccès libreIxodes ricinus L. : mast cells, basophils and eosinophils in the sequence of cellular events in the skin of infested or re-infested rabbits(1982)
; Fivaz, V.Some information on acquired resistance of rabbits against Ixodes ricinus ticks has been obtained by comparing tissue changes and cellular infiltration during 2 successive infestations, with particular emphasis on the cells involved in immediate type hypersensitivity reactions: mast cells, basophils and eosinophils. More degranulated mast cells were observed in tick lesions 2 h after the beginning of re-infestation than in the first infestation and more degranulated basophils were present on the 5th day of re-infestation. Numerous eosinophils infiltrated the dermis, especially on the 5th day of the second infestation. - PublicationAccès libreIxodes ricinus Tick Lipocalins: Identification, Cloning, Phylogenetic Analysis and Biochemical Characterization(2008)
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Methodology/Principal Findings : Screening a cDNA library in association with RT-PCR and RACE methodologies allowed us to identify 14 new lipocalin genes in the salivary glands of the Ixodes ricinus hard tick. A computational in-depth structural analysis confirmed that LIRs belong to the lipocalin family. These proteins were called LIR for “Lipocalin from I. ricinus” and numbered from 1 to 14 (LIR1 to LIR14). According to their percentage identity/similarity, LIR proteins may be assigned to 6 distinct phylogenetic groups. The mature proteins have calculated pM and pI varying from 21.8 kDa to 37.2 kDa and from 4.45 to 9.57 respectively. In a western blot analysis, all recombinant LIRs appeared as a series of thin bands at 50–70 kDa, suggesting extensive glycosylation, which was experimentally confirmed by treatment with N-glycosidase F. In addition, the in vivo expression analysis of LIRs in I. ricinus, examined by RT-PCR, showed homogeneous expression profiles for certain phylogenetic groups and relatively heterogeneous profiles for other groups. Finally, we demonstrated that LIR6 codes for a protein that specifically binds leukotriene B4.
Conclusions/Significance : This work confirms that, regarding their biochemical properties, expression profile, and sequence signature, lipocalins in Ixodes hard tick genus, and more specifically in the Ixodes ricinus species, are segregated into distinct phylogenetic groups suggesting potential distinct function. This was particularly demonstrated by the ability of LIR6 to scavenge leukotriene B4. The other LIRs did not bind any of the ligands tested, such as 5-hydroxytryptamine, ADP, norepinephrine, platelet activating factor, prostaglandins D2 and E2, and finally leukotrienes B4 and C4. - PublicationAccès libreCytokines (IL-4 and IFN-γ) and antibodies (IgE and IgG2a) produced in mice infected with Borrelia burgdorferi sensu stricto via nymphs of Ixodes ricinus ticks or syringe inoculations(2000)
- PublicationAccès libreIsolation of Ixodes ricinus salivary gland mRNA encoding factors induced during blood feeding(2002)
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