Logo du site
  • English
  • Français
  • Se connecter
Logo du site
  • English
  • Français
  • Se connecter
  1. Accueil
  2. Université de Neuchâtel
  3. Publications
  4. Using DNA-barcoding for sorting out protest species complexes:: A case study of the <i>Nebela tincta–collaris–bohemica</i> group (Amoebozoa; Arcellinida, Hyalospheniidae)
 
  • Details
Options
Vignette d'image

Using DNA-barcoding for sorting out protest species complexes:: A case study of the <i>Nebela tincta–collaris–bohemica</i> group (Amoebozoa; Arcellinida, Hyalospheniidae)

Auteur(s)
Kosakyan, Anush 
Institut de biologie 
Gomaa, Fatma
Mitchell, Edward 
Institut de biologie 
Heger, Thierry J.
Lara, Enrique 
Institut de biologie 
In
European Journal of Protistology, 2013/49//222-237
Mots-clés
  • Barcoding
  • COI
  • <i>Nebela tincta–collaris–bohemica</i>
  • Species complex
  • Taxonomy
  • Barcoding

  • COI

  • <i>Nebela tincta–coll...

  • Species complex

  • Taxonomy

Résumé
Species identification by means of morphology is often problematic in protists. <i>Nebela tincta–collaris–bohemica</i> (Arcellinida) is a species complex of small to medium-sized (ca.100 μm) testate amoebae common in peat bogs and forest soils. The taxonomic validity of characters used to define species within this group is debated and causes confusion in studies of biogeography, and applications in palaeoecology. <br> We examined the relationship between morphological and genetic diversity within this species complex by combined analyses of light microscopy imaging and Cytochrome Oxidase Subunit 1(COI) sequences obtained from the same individual amoeba cells. Our goals were (1) to clarify the taxonomy and the phylogenetic relationships within this group, and (2) to evaluate if individual genotypes corresponded to specific morphotypes and the extent of phenotypic plasticity. <br> We show here that small variations in test morphology that have been often overlooked by traditional taxonomy correspond to distinct haplotypes. We therefore revise the taxonomy of the group. We redefine <i>Nebela tincta</i> (Leidy) Kosakyan et Lara and <i>N. collaris</i> (Ehrenberg 1848) Kosakyan et Gomaa, change <i>N. tincta</i> var. <i>rotunda</i> Penard to <i>N. rotunda</i> (Penard 1890), describe three new species: <i>N. guttata</i> n. sp. Kosakyan et Lara, <i>N. pechorensis</i> n. sp. Kosakyan et Mitchell, and <i>N. aliciae</i> n. sp. Mitchell et Lara.
Identifiants
https://libra.unine.ch/handle/123456789/9408
_
10.1016/j.ejop.2012.08.006
Type de publication
journal article
Dossier(s) à télécharger
 main article: Kosakyan_Anush-Using_DNA-barcoding_for_sorting_out_protist-20130326.pdf (1.67 MB)
google-scholar
Présentation du portailGuide d'utilisationStratégie Open AccessDirective Open Access La recherche à l'UniNE Open Access ORCIDNouveautés

Service information scientifique & bibliothèques
Rue Emile-Argand 11
2000 Neuchâtel
contact.libra@unine.ch

Propulsé par DSpace, DSpace-CRIS & 4Science | v2022.02.00