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The <i>Anopheles gambiae</i> Odorant Binding Protein 1 (AgamOBP1) Mediates Indole Recognition in the Antennae of Female Mosquitoes
Auteur(s)
Biessmann, Harald
Andronopoulou, Evi
Biessmann, Max R.
Douris, Vassilis
Dimitratos, Spiros D.
Eliopoulos, Elias
Iatrou, Kostas
Justice, Robin W.
Kröber, Thomas
Marinotti, Osvaldo
Tsitoura, Panagiota
Woods, Daniel F.
Walter, Marika F.
Date de parution
2010
In
PloS One, Public Library of Science (PLoS), 2010/5/3/e9471 1-8
Résumé
Haematophagous insects are frequently carriers of parasitic diseases, including malaria. The mosquito <i>Anopheles gambiae</i> is the major vector of malaria in sub-Saharan Africa and is thus responsible for thousands of deaths daily. Although the role of olfaction in <i>A. gambiae</i> host detection has been demonstrated, little is known about the combinations of ligands and odorant binding proteins (OBPs) that can produce specific odor-related responses <i>in vivo</i>. We identified a ligand, indole, for an <i>A. gambiae</i> odorant binding protein, AgamOBP1, modeled the interaction in silico and confirmed the interaction using biochemical assays. RNAi-mediated gene silencing coupled with electrophysiological analyses confirmed that AgamOBP1 binds indole in <i>A. gambiae</i> and that the antennal receptor cells do not respond to indole in the absence of AgamOBP1. This case represents the first documented instance of a specific <i>A. gambiae</i> OBP–ligand pairing combination, demonstrates the significance of OBPs in odor recognition, and can be expanded to the identification of other ligands for OBPs of <i>Anopheles</i> and other medically important insects.
Identifiants
Type de publication
journal article
