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Identification of two gtp-binding proteins in the chloroplast protein import machinery
Auteur(s)
Date de parution
1994
In
Science
Vol.
5187
No
266
De la page
1035
A la page
1039
Résumé
Two of four proteins that associated with translocation intermediates during protein import across the outer chloroplast envelope membrane were identified as guanosine triphosphate (GTP)-binding proteins. Both proteins are integral membrane proteins of the outer chloroplast membrane, and both are partially exposed on the chloroplast surface where they were accessible to thermolysin digestion. Engagement of the outer membrane's import machinery by an import substrate was inhibited by slowly hydrolyzable or non-hydrolyzable GTP analogs. Thus, these GTP-binding proteins may function in protein import into chloroplasts.
Identifiants
Type de publication
journal article
