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Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles
Auteur(s)
Vidi, Pierre-Alexandre
Baginsky, Sacha
Austin, Jotham R.
Csucs, Gabor
Dörmann, Peter
Bréhélin, Claire
Date de parution
2006
In
The Journal of Biological Chemistry, 2006/281/11225-11234
Résumé
Chloroplasts contain lipoprotein particles termed plastoglobules. Plastoglobules are generally believed to have little function beyond lipid storage. Here we report on the identification of plastoglobule proteins using mass spectrometry methods in <i>Arabidopsis thaliana</i>. We demonstrate specific plastoglobule association of members of the plastid lipid-associated proteins/fibrillin family as well as known metabolic enzymes, including the tocopherol cyclase (VTE1), a key enzyme of tocopherol (vitamin E) synthesis. Moreover, comparative analysis of chloroplast membrane fractions shows that plastoglobules are a site of vitamin E accumulation in chloroplasts. Thus, in addition to their lipid storage function, we propose that plastoglobules are metabolically active, taking part in tocopherol synthesis and likely other pathways.
Identifiants
Type de publication
journal article
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