Options
Identification of Plastoglobules as a Site of Carotenoid Cleavage
Auteur(s)
Rottet, Sarah
Devillers, Julie
Glauser, Gaétan
Besagni, Céline
In
Frontiers in Plant Science, Frontiers Media S.A., 2016/7//Article 1855
Résumé
Carotenoids play an essential role in light harvesting and protection from excess light. During chloroplast senescence carotenoids are released from their binding proteins and are eventually metabolized. Carotenoid cleavage dioxygenase 4 (CCD4) is involved in carotenoid breakdown in senescing leaf and desiccating seed, and is part of the proteome of plastoglobules (PG), which are thylakoid-associated lipid droplets. Here, we demonstrate that CCD4 is functionally active in PG. Leaves of Arabidopsis thaliana ccd4 mutants constitutively expressing CCD4 fused to yellow fluorescent protein showed strong fluorescence in PG and reduced carotenoid levels upon dark- induced senescence. Lipidome-wide analysis indicated that ß-carotene, lutein, and violaxanthin were the principle substrates of CCD4 in vivo and were cleaved in senescing chloroplasts. Moreover, carotenoids were shown to accumulate in PG of <i>ccd4</i> mutant plants during senescence, indicating translocation of carotenoids to PG prior to degradation.
Identifiants
Type de publication
journal article
Dossier(s) à télécharger
