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  4. Dimerization of Toc-GTPases at the Chloroplast Protein Import Machinery
 
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Dimerization of Toc-GTPases at the Chloroplast Protein Import Machinery

Auteur(s)
Hiltbrunner, Andreas
Kessler, Félix 
Institut de biologie 
Weibel, Petra 
Institut de biologie 
Date de parution
2003
In
The Journal of Biological Chemistry, 2003/278/37321-37329
Résumé
Import of chloroplast precursor proteins is controlled by the coordinate action of two homologous GTPases, Toc159 and Toc33, located at the cytosol-outer membrane interface. Recent studies in <i>Arabidopsis</i> showed that the cytosolic form of the precursor binding protein Toc159 is targeted to its receptor at the import machinery, Toc33, via heterodimerization of their GTP-binding domains. Toc33 may also form GDP-bound homodimers, as suggested by the crystal structure of its pea ortholog. Moreover, the structural data suggested that arginine 130 (Arg<sup>130</sup>) of <i>Arabidopsis</i> Toc33 may function as a GTPase-activating "arginine-finger" at the other monomer in the Toc33 dimer. Here, we demonstrate that Arg<sup>130</sup> of Toc33 does not function as an Arginine-finger. A mutant, Toc33-R130A, binds and hydrolyzes GTP like the wild type. However, we demonstrate that Arg<sup>130</sup> is involved in both homodimerization of Toc33 and in heterodimerization with the GTP-binding domain of Toc159. The dependence of Toc33 homodimerization on Arg<sup>130</sup> is mutual, requiring the presence of Arg<sup>130</sup> at both monomers. As the GTPase is not activated by dimerization, it may be activated independently at either monomer, possibly even before dimerization. Independent regulation of GTPase activity may serve to coordinate the interactions of the GTPases during the import of proteins into the chloroplast.
Identifiants
https://libra.unine.ch/handle/123456789/18602
_
10.1074/jbc.M305946200
Type de publication
journal article
Dossier(s) à télécharger
 main article: Weibel_Petra_-_Dimerization_of_Toc-GTPases_20060725.pdf (744.69 KB)
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