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  4. Identification of genes expressed during the compatible interaction of grapevine with <i>Plasmopara viticola</i> through suppression subtractive hybridization (SSH)
 
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Identification of genes expressed during the compatible interaction of grapevine with <i>Plasmopara viticola</i> through suppression subtractive hybridization (SSH)

Auteur(s)
Legay, Guillaume
Marouf, Elaheh
Berger, Dave
Neuhaus, Jean-Marc 
Institut de biologie 
Mauch-Mani, Brigitte 
Institut de biologie 
Slaughter, Ana R.
Date de parution
2011
In
European Journal of Plant Pathology, Springer, 2011/129/2/281-301
Mots-clés
  • Compatible interaction
  • Reverse transcriptase quantitative real-time PCR
  • <i>Plasmopara viticola</i>
  • Suppression subtractive hybridization
  • <i>Vitis vinifera</i>
  • Compatible interactio...

  • Reverse transcriptase...

  • <i>Plasmopara viticol...

  • Suppression subtracti...

  • <i>Vitis vinifera</i>...

Résumé
Grapevine (<i>Vitis vinifera</i>) is the most widely cultivated and economically important fruit crop, but is susceptible to a large number of diseases. Downy mildew, caused by the obligate biotrophic oomycete pathogen <i>Plasmopara viticola</i>, is a common disease present in all regions where vines are cultivated. We used suppression subtractive hybridization (SSH) to generate two cDNA libraries enriched for transcripts induced and repressed, respectively, in the susceptible grapevine cultivar Chasselas 24 h after inoculation with <i>P. viticola</i>. Differential screening on glass slide microarrays yielded over 800 putative genes that were up-regulated in response to <i>P. viticola</i> infection and over 200 that were down-regulated. One hundred and ninety four of these, were sequenced, identified and functionally categorised. Transcript abundance of twelve genes over a 48 h time course was examined by reverse transcriptase quantitative real-time PCR (RT-qPCR). Ten of these genes were induced/enhanced by <i>P. viticola</i> challenge, confirming the results of the SSH. The vast majority of the genes identified are related to defence. Interestingly, many genes involved in photosynthesis were down-regulated.
Identifiants
https://libra.unine.ch/handle/123456789/11590
_
10.1007/s10658-010-9676-z
Type de publication
journal article
Dossier(s) à télécharger
 main article: Legay_Guillaume_-_Identification_of_genes_expressed_during_the_20110718.pdf (849.52 KB)
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