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  4. Mycamoeba gemmipara nov. gen., nov. sp., the First Cultured Member of the Environmental Dermamoebidae Clade LKM74 and its Unusual Life Cycle
 
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Mycamoeba gemmipara nov. gen., nov. sp., the First Cultured Member of the Environmental Dermamoebidae Clade LKM74 and its Unusual Life Cycle

Auteur(s)
Blandenier, Quentin
Seppey, Christophe 
Institut de biologie 
Singer, David 
Institut de biologie 
Vlimant, Michèle 
Institut de biologie 
Duckert, Clément
Lara, Enrique 
Institut de biologie 
Date de parution
2016-8-20
In
J Eukaryot Microbiol.
No
0
De la page
1
A la page
9
Revu par les pairs
1
Mots-clés
  • Budding

  • Discosea

  • Eukaryotic diversity

  • Fungus

  • Longamoebia

  • high throughput seque...

  • ribosomal genes

  • serial dilution

  • yeast

Résumé
Since the first environmental DNA surveys, entire groups of sequences called “environmental clades” did not have any cultured representative. LKM74 is an amoebozoan clade affiliated to Dermamoebidae, whose presence is pervasively reported in soil and freshwater. We obtained an isolate from soil that we assigned to LKM74 by molecular phylogeny, close related to freshwater clones. We described Mycamoeba gemmipara based on observations made with light- and transmission electron microscopy. It is an extremely small amoeba with typical lingulate shape. Unlike other Dermamoebidae, it lacked ornamentation on its cell membrane, and condensed chromatin formed characteristic patterns in the nucleus. M. gemmipara displayed a unique life cycle: trophozoites formed walled coccoid stages which grew through successive buddings and developed into branched structures holding cysts. These structures, measuring hundreds of micrometres, are built as the exclusive product of osmotrophic feeding. In order to demonstrate that M. gemmipara is a genuine soil inhabitant, we screened its presence in an environmental soil DNA diversity survey performed on an experimental setup where pig cadavers were left to decompose in soils in order to follow changes in eukaryotic communities. M. gemmipara was present in all samples, although related reads were uncommon underneath the cadaver.
URI
https://libra.unine.ch/handle/123456789/24889
DOI
10.1111/jeu.12357/abstract;jsessionid=51658871C960A7F062770A1080E523A4.f04t01
Autre version
http://onlinelibrary.wiley.com/doi/10.1111/jeu.12357/abstract;jsessionid=51658871C960A7F062770A1080E523A4.f04t01
Type de publication
Resource Types::text::journal::journal article
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