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  4. The C-terminal tetrapeptide of phaseolin is sufficient to target green fluorescent protein to the vacuole
 
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The C-terminal tetrapeptide of phaseolin is sufficient to target green fluorescent protein to the vacuole

Auteur(s)
Frigerio, Lorenzo
Editeur(s)
Foresti, Ombretta
Hernández Felipe, Doramys
Neuhaus, Jean-Marc 
Institut de biologie 
Vitale, Alessandro
Date de parution
2001
In
Journal of Plant Physiology, 2001/158/499-503
Mots-clés
  • plant secretory pathw...

  • vacuolar sorting

  • phaseolin

  • green fluorescent pro...

Résumé
Phaseolin is a vacuolar storage glycoprotein synthesized as a precursor with a short C-terminal propeptide. We have recently shown that deletion of the last four C-terminal amino acids (AFVY, which are part of, or constitute the propeptide) abolishes vacuolar targeting, causing phaseolin to be secreted. Here we provide biochemical and microscopical evidence that the AFVY tetrapeptide, when fused to a secreted version of green fluorescent protein (GFP), inhibits GFP secretion and leads to its accumulation in vacuoles, where it is processed. This demonstrates that the tetrapeptide contains sufficient information for vacuolar sorting.
URI
https://libra.unine.ch/handle/123456789/18932
DOI
10.1078/0176-1617-00362
Autre version
http://dx.doi.org/10.1078/0176-1617-00362
Type de publication
Resource Types::text::journal::journal article
Dossier(s) à télécharger
 main article: 1_Neuhaus_Jean-Marc_-_The_C-terminal_tetrapeptide_of_phaseolin_20060310.pdf (255.66 KB)
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