Logo du site
  • English
  • Français
  • Se connecter
Logo du site
  • English
  • Français
  • Se connecter
  1. Accueil
  2. Université de Neuchâtel
  3. Publications
  4. Plastid Proteome Assembly without Toc159: Photosynthetic Protein Import and Accumulation of <i>N</i>-Acetylated Plastid Precursor Proteins
 
  • Details
Options
Vignette d'image

Plastid Proteome Assembly without Toc159: Photosynthetic Protein Import and Accumulation of <i>N</i>-Acetylated Plastid Precursor Proteins

Auteur(s)
Bischof, Sylvain
Editeur(s)
Baerenfaller, Katja
Wildhaber, Thomas
Troesch, Raphael
Vidi, Pierre-Alexandre
Roschitzki, Bernd
Hirsch-Hoffmann, Matthias
Hennig, Lars
Kessler, FĂ©lix 
Institut de biologie 
Gruissem, Wilhelm
Baginsky, Sacha
In
The Plant Cell, American Society of Plant Biologists, 2011/23/11/3911-3928
Résumé
Import of nuclear-encoded precursor proteins from the cytosol is an essential step in chloroplast biogenesis that is mediated by protein translocon complexes at the inner and outer envelope membrane (TOC). Toc159 is thought to be the main receptor for photosynthetic proteins, but lacking a large-scale systems approach, this hypothesis has only been tested for a handful of photosynthetic and nonphotosynthetic proteins. To assess Toc159 precursor specificity, we quantitatively analyzed the accumulation of plastid proteins in two mutant lines deficient in this receptor. Parallel genome-wide transcript profiling allowed us to discern the consequences of impaired protein import from systemic transcriptional responses that contribute to the loss of photosynthetic capacity. On this basis, we defined putative Toc159-independent and Toc159- dependent precursor proteins. Many photosynthetic proteins accumulate in Toc159- deficient plastids, and, surprisingly, several distinct metabolic pathways are negatively affected by Toc159 depletion. Lack of Toc159 furthermore affects several proteins that accumulate as unprocessed <i>N</i>-acetylated precursor proteins outside of plastids. Together, our data show an unexpected client protein promiscuity of Toc159 that requires a far more differentiated view of Toc159 receptor function and regulation of plastid protein import, in which cytosolic Met removal followed by N-terminal acetylation of precursors emerges as an additional regulatory step.
URI
https://libra.unine.ch/handle/123456789/5145
DOI
10.1105/tpc.111.092882
Autre version
http://dx.doi.org/10.1105/tpc.111.092882
Type de publication
Resource Types::text::journal::journal article
Dossier(s) à télécharger
 main article: Bischof_S.-Plastid_proteom-20170222160014-ZZ.pdf (4.43 MB)
google-scholar
Présentation du portailGuide d'utilisationStratégie Open AccessDirective Open Access La recherche à l'UniNE Open Access ORCID

Adresse:
UniNE, Service information scientifique & bibliothèques
Rue Emile-Argand 11
2000 Neuchâtel

Construit avec Logiciel DSpace-CRIS Maintenu et optimiser par 4Sciences

  • Paramètres des tĂ©moins de connexion
  • Politique de protection de la vie privĂ©e
  • Licence de l'utilisateur final