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Brossard, Michel

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Brossard, Michel
Affiliation principale
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https://libra.unine.ch/handle/123456789/308

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  • Publication
    Accès libre
    Th2 polarization of the immune response of BALB/c mice to Ixodes ricinus instars, importance of several antigens in activation of specific Th2 subpopulations
    (2001)
    Mejri, Naceur
    ;
    Franscini, Nicola
    ;
    Rutti, Bernard
    ;
    Brossard, Michel 
    BALB/c mice were infested with Ixodes ricinus larvae, nymphs or adults. Expression of IL-4 and IFN-γ mRNA in axillary and brachial draining lymph node cells were measured by competitive quantitative reverse transcription-polymerase chain reaction 9 days after the beginning of primary-infestation. IL-4 mRNA was always higher than that of IFN-γ mRNA for all tick instars. Moreover, IL-4 mRNA expression progressively increased during nymphal primary-infestation with a high burst of expression 7 days after the beginning of infestation. No evolution of IFN-γ mRNA expression was detected. Draining lymph node cells of infested BALB/c produced higher level of IL-4 than IFN-γ following in vitro restimulation with adult tick saliva, salivary gland extract (SGE) or with five selected different chromatographic fractions of SGE. Anti-tick IgG1 antibodies but no IgG2a were detected in BALB/c pluri-infested with I. ricinus nymphs, which confirmed the Th2 polarization of the immune response.