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Comparison of gene targeting efficiencies in two mosses suggests that it is a conserved feature of Bryophyte transformation
Auteur(s)
Trouiller, Bénédicte
Charlot, Florence
Choinard, Sandrine
Schaefer, Didier G.
Nogué, Fabien
Date de parution
2007
In
Plant Systematics and Evolution, Springer, 2007/29/10/1591-1598
Résumé
The moss, <i>Physcomitrella patens</i>, is a novel tool in plant functional genomics due to its exceptionally high gene targeting efficiency that is so far unique for plants. To determine if this high gene targeting efficiency is exclusive to <i>P. patens</i> or if it is a common feature to mosses, we estimated gene-targeting efficiency in another moss, <i>Ceratodon purpureus</i>. We transformed both mosses with replacement vectors corresponding to the adenine phosphoribosyl transferase (<i>APT</i>) reporter gene. We achieved a gene targeting efficiency of 20.8% for <i>P. patens</i> and 1.05% for <i>C. purpureus</i>. Our findings support the hypothesis that efficient gene targeting could be a general mechanism of Bryophyte transformation.
Identifiants
Type de publication
journal article
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