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How elevated pCO<sup>2</sup> modifies total and metabolically active bacterial communities in the rhizosphere of two perennial grasses grown under field conditions
Auteur(s)
Jossi, Maryline
Fromin, Nathalie
Tarnawski, Sonia
Kohler, Florian
Hamelin, Jérôme
Date de parution
2006
In
FEMS Microbiology Ecology, Blackwell, 2006/55/3/339-350
Résumé
The response of total (DNA-based analysis) and active (RNA-based analysis) bacterial communities to a pCO<sup>2</sup> increase under field conditions was assessed using two perennial grasses: the nitrophilic <i>Lolium perenne</i> and the oligonitrophilic <i>Molinia coerulea</i>. PCR- and reverse transcriptase-PCR denaturing gradient gel electrophoresis analysis of 16S rRNA genes generated contrasting profiles. The pCO<sup>2</sup> increase influenced mainly the active and root-associated component of the bacterial community. Bacterial groups responsive to the pCO<sup>2</sup> increase were identified by sequencing of corresponding denaturing gradient gel electrophoresis bands. About 50% of retrieved sequences were affiliated to <i>Proteobacteria</i>. Our data suggest that <i>Actinobacteria</i> in soil and Myxococcales (<i>Deltaproteobacteria</i>) in root are stimulated under elevated pCO<sup>2</sup>.
Identifiants
Type de publication
journal article
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