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DNA extraction from <i>Ascaris suum</i> muscle tissue
Auteur(s)
Di Mito, Carmela
Date de parution
1998
In
Parasitology Research, Springer, 1998/84/7/596-597
Résumé
A new method for the extraction of DNA from <i>Ascaris suum</i> muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
Identifiants
Type de publication
journal article
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