Logo du site
  • English
  • Français
  • Se connecter
Logo du site
  • English
  • Français
  • Se connecter
  1. Accueil
  2. Université de Neuchâtel
  3. Publications
  4. Sensitive and selective quantification of free and total malondialdehyde in plasma using UHPLC-HRMS
 
  • Details
Options
Vignette d'image

Sensitive and selective quantification of free and total malondialdehyde in plasma using UHPLC-HRMS

Auteur(s)
Mendonça, Rute 
Institut de biologie 
Gning, Ophélie 
Institut de biologie 
Di Cesare, Claudia 
Institut de biologie 
Lachat, Laurence 
Institut de chimie 
Bennett, Nigel C
Helfenstein, Fabrice 
Institut de biologie 
Glauser, Gaëtan 
Institut de biologie 
Date de parution
2017-7-10
In
Journal of Lipid Research
No
in press
De la page
999
A la page
999
Revu par les pairs
1
Mots-clés
  • Fatty acids
  • Oxidation
  • Lipid peroxidation
  • Malondialdehyde
  • Fatty acids

  • Oxidation

  • Lipid peroxidation

  • Malondialdehyde

Résumé
Quantification of malondialdehyde (MDA) as a marker of lipid peroxidation is relevant for many research fields. We describe a new sensitive and selective method to measure free and total plasmatic MDA, using derivatization with 2,4-dinitrophenylhydrazine (DNPH) and ultrahigh performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Free and total MDA were extracted from minute sample amounts (10 μL) using acidic precipitation and alkaline hydrolysis followed by acidic precipitation, respectively. Derivatization was completed within 10 min at room temperature, and the excess DNPH discarded by liquid-liquid extraction. Quantification was achieved by internal standardization using dideuterated MDA as internal standard. The method lowest limit of quantification (LLOQ) was 100 nM and linearity spanned > 3 orders of magnitude. Intra- and inter-day precisions for total MDA were 2.9% and 3.0%, respectively, and those for free MDA were 12.8% and 24.9%, respectively. Accuracy was 101% and 107% at low and high concentrations, respectively. In human plasma, free MDA levels were 120 nM (SD 36.26) and total MDA levels were 6.7 μM (SD 0.46). In addition, we show the applicability of this method to measure MDA plasma levels from a variety of animal species, making it invaluable to scientists in various fields.
Identifiants
https://libra.unine.ch/handle/123456789/25928
Type de publication
journal article
Dossier(s) à télécharger
 main article: PIIS0022227520339626.pdf (642.98 KB)
google-scholar
Présentation du portailGuide d'utilisationStratégie Open AccessDirective Open Access La recherche à l'UniNE Open Access ORCIDNouveautés

Service information scientifique & bibliothèques
Rue Emile-Argand 11
2000 Neuchâtel
contact.libra@unine.ch

Propulsé par DSpace, DSpace-CRIS & 4Science | v2022.02.00