Logo du site
  • English
  • Français
  • Se connecter
Logo du site
  • English
  • Français
  • Se connecter
  1. Accueil
  2. Université de Neuchâtel
  3. Publications
  4. Protein expression profile of Gasterophilus intestinalis larvae causing horse gastric myiasis and characterization of horse immune reaction
 
  • Details
Options
Vignette d'image

Protein expression profile of Gasterophilus intestinalis larvae causing horse gastric myiasis and characterization of horse immune reaction

Auteur(s)
Roelfstra, Liselore
Deeg, Cornelia A.
Hauck, Stefanie M.
Buse, Christina
Membrez, Mathieu
Betschart, Bruno 
Institut de biologie 
Pfister, Kurt 
Institut de biologie 
Date de parution
2009
In
Parasites & Vectors, BioMed Central, 2009/2/6/1-8
Résumé
<b>Background</b> Little information is available on the immunological aspect of parasitic Gasterophilus intestinalis (Diptera, Oestridae) larvae causing horse gastric myiasis. The objectives of this research were to analyze the protein content of larval crude extracts of the migrating second and third larvae (L2 and L3) of G. intestinalis in order to characterize the immune response of horses. <br> <b>Results</b> The proteomic profile of L2 and L3, investigated by using one and two dimensional approaches, revealed a migration pattern specific to each larval stage. Furthermore, Western blots were performed with horse sera and with sera of Balb/c mice immunised with the larval crude extracts of L2 or L3, revealing a different immune reaction in naturally infected horses vs. artificially induced immune reaction in mice. The comparisons of the immunoblot profiles demonstrate that the stage L2 is more immunogenic than the stage L3 most likely as an effect of the highest enzymatic production of L2 while migrating through the host tissues. Fifteen proteins were identified by mass spectrometry. <br> <b>Conclusion</b> This work provides further information into the understanding of the interaction between G. intestinalis and their host and by contributing a novel scheme of the proteomic profile of the main larval stages.
Identifiants
https://libra.unine.ch/handle/123456789/15210
_
10.1186/1756-3305-2-6
Type de publication
journal article
Dossier(s) à télécharger
 main article: Roelfstra_Liselore_-_Protein_expression_profile_of_Gasterophilus_20090702.pdf (878.41 KB)
google-scholar
Présentation du portailGuide d'utilisationStratégie Open AccessDirective Open Access La recherche à l'UniNE Open Access ORCIDNouveautés

Service information scientifique & bibliothèques
Rue Emile-Argand 11
2000 Neuchâtel
contact.libra@unine.ch

Propulsé par DSpace, DSpace-CRIS & 4Science | v2022.02.00