Logo du site
  • English
  • Français
  • Se connecter
Logo du site
  • English
  • Français
  • Se connecter
  1. Accueil
  2. Université de Neuchâtel
  3. Publications
  4. Environmental DNA COI barcoding for quantitative analysis of protists communities: A test using the <i>Nebela collaris</i> complex (Amoebozoa;Arcellinida; Hyalospheniidae)
 
  • Details
Options
Vignette d'image

Environmental DNA COI barcoding for quantitative analysis of protists communities: A test using the <i>Nebela collaris</i> complex (Amoebozoa;Arcellinida; Hyalospheniidae)

Auteur(s)
Kosakyan, Anush 
Institut de biologie 
Mulot, Matthieu 
Institut de biologie 
Mitchell, Edward 
Institut de biologie 
Lara, Enrique 
Institut de biologie 
In
European Journal of Protistology, Elsevier, 2015/51/4/311-320
Mots-clés
  • Community ecology
  • Environmental DNA
  • Metabarcoding
  • Protist diversity
  • <i>Sphagnum</i> peatlands
  • Testate amoebae
  • Community ecology

  • Environmental DNA

  • Metabarcoding

  • Protist diversity

  • <i>Sphagnum</i> peatl...

  • Testate amoebae

Résumé
Environmental DNA surveys are used for screening eukaryotic diversity. However, it is unclear how quantitative this approach is and to what extent results from environmental DNA studies can be used for ecological studies requiring quantitative data. Mitochondrial cytochrome oxidase (COI) is used for species-level taxonomic studies of testate amoebae and should allow assessing the community composition from environmental samples, thus bypassing biases due to morphological identification. We tested this using a COI clone library approach and focusing on the <i>Nebela collaris</i> complex. Comparisons with direct microscopy counts showed that the COI clone library diversity data matched the morphologically identified taxa, and that community com-position estimates using the two approaches were similar. However, this correlation was improved when microscopy counts were corrected for biovolume. Higher correlation with biovolume-corrected community data suggests that COI clone library data matches the ratio of mitochondria and that within closely-related taxa the density of mitochondria per unit biovolume is approximately constant. Further developments of this metabarcoding approach including quantifying the mitochondrial density among closely-related taxa, experiments on other taxonomic groups and using high throughput sequencing should make if possible to quantitatively estimate community composition of different groups, which would be invaluable for microbial food webs studies.
Identifiants
https://libra.unine.ch/handle/123456789/7034
_
10.1016/j.ejop.2015.06.005
Type de publication
journal article
Dossier(s) à télécharger
 main article: Kosakyan_A.-Environmental_DNA-20150819.pdf (2.12 MB)
google-scholar
Présentation du portailGuide d'utilisationStratégie Open AccessDirective Open Access La recherche à l'UniNE Open Access ORCIDNouveautés

Service information scientifique & bibliothèques
Rue Emile-Argand 11
2000 Neuchâtel
contact.libra@unine.ch

Propulsé par DSpace, DSpace-CRIS & 4Science | v2022.02.00