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Glauser, Gaëtan

Nom
Glauser, Gaëtan
Affiliation principale
Email
gaetan.glauser@unine.ch
Identifiants
https://libra.unine.ch/handle/123456789/369
_
0000-0002-0983-8614

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  • Publication
    Métadonnées seulement
    Ultra-high pressure liquid chromatography-mass spectrometry for plant metabolomics: A systematic comparison of high-resolution quadrupole-time-of-flight and single stage Orbitrap mass spectrometers
    (2013)
    Glauser, Gaëtan 
    ;
    Veyrat, Nathalie 
    ;
    Rochat, B.
    ;
    Wolfender, Jean-Luc
    ;
    Turlings, Ted 
    The response of Arabidopsis to stress caused by mechanical wounding was chosen as a model to compare the performances of high resolution quadrupole-time-of-flight (Q-TOF) and single stage Orbitrap (Exactive Plus) mass spectrometers in untargeted metabolomics. Both instruments were coupled to ultra-high pressure liquid chromatography (UHPLC) systems set under identical conditions. The experiment was divided in two steps: the first analyses involved sixteen unwounded plants, half of which were spiked with pure standards that are not present in Arabidopsis. The second analyses compared the metabolomes of mechanically wounded plants to unwounded plants. Data from both systems were extracted using the same feature detection software and submitted to unsupervised and supervised multivariate analysis methods. Both mass spectrometers were compared in terms of number and identity of detected features, capacity to discriminate between samples, repeatability and sensitivity. Although analytical variability was lower for the UHPLC-Q-TOF, generally the results for the two detectors were quite similar, both of them proving to be highly efficient at detecting even subtle differences between plant groups. Overall, sensitivity was found to be comparable, although the Exactive Plus Orbitrap provided slightly lower detection limits for specific compounds. Finally, to evaluate the potential of the two mass spectrometers for the identification of unknown markers, mass and spectral accuracies were calculated on selected identified compounds. While both instruments showed excellent mass accuracy (
  • Publication
    Métadonnées seulement
    Rapid profiling of intact glucosinolates in
    (2012)
    Glauser, Gaëtan 
    ;
    Schweizer, F.
    ;
    Turlings, Ted 
    ;
    Reymond, P.
    Introduction The analysis of glucosinolates (GS) is traditionally performed by reverse-phase liquid chromatography coupled to ultraviolet detection after a time-consuming desulphation step, which is required for increased retention. Simpler and more efficient alternative methods that can shorten both sample preparation and analysis are much needed. Objective To evaluate the feasibility of using ultrahigh-pressure liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS) for the rapid profiling of intact GS. Methodology A simple and short extraction of GS from Arabidopsis thaliana leaves was developed. Four sub-2?mu m reverse-phase columns were tested for the rapid separation of these polar compounds using formic acid as the chromatographic additive. High-resolution QTOFMS was used to detect and identify GS. Results A novel charged surface hybrid (CSH) column was found to provide excellent retention and separation of GS within a total running time of 11?min. Twenty-one GS could be identified based on their accurate mass as well as isotopic and fragmentation patterns. The method was applied to determine the changes in GS content that occur after herbivory in Arabidopsis. In addition, we evaluated its applicability to the profiling of other Brassicaceae species. Conclusion The method developed can profile the full range of GS, including the most polar ones, in a shorter time than previous methods, and is highly compatible with mass spectrometric detection. Copyright (c) 2012 John Wiley & Sons, Ltd.