Vignette d'image

Junier, Pilar

Nom
Junier, Pilar
Affiliation principale
Fonction
Professeure assistante
Email
pilar.junier@unine.ch
Identifiants
https://libra.unine.ch/handle/123456789/362

Résultat de la recherche

Filtres

2
2
2
Réinitialiser les filtres

Paramètres

Voici les éléments 1 - 2 sur 2
  • Publication
    Accès libre
    Genetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, psbA and ntcA sequence analyses
    (2007)
    Junier, Pilar 
    ;
    Witzel, Karl-Paul
    ;
    Hadas, Ora
    The genetic diversity of cyanobacterial communities was studied at various depths in Lake Kinneret (Israel). Denaturing gradient gel electrophoresis (DGGE) of specific 16S rRNA gene PCR products showed significant differences in the cyanobacterial community structure between epi- and hypolimnetic waters. Sequences of clone libraries prepared from 16S rRNA gene PCR products from epi- and hypolimnion revealed the presence of at least 11 different groups of cyanobacteria. Clones related to the unicellular cyanobacteria (Chroococcales and picocyanobacteria) dominated the clone libraries from both depths. New primers to amplify the gene coding for the photosystem II reaction centre (psbA) and the nitrogen regulator gene (ntcA) of cyanobacteria were developed and used for further characterization of the cyanobacterial communities from the lake. Sequences of psbA amplicons clustered with those from 2 different groups of marine Synechococcus and Chroococcales. Cloned ntcA amplicons from the lake were closely related and did not cluster with sequences from cultured cyanobacteria or other environmental sequences from this gene. All the molecular markers analyzed here showed similarity to sequences from some groups of cyanobacteria in the lake and those so far found in marine habitats.
  • Publication
    Accès libre
    Effect of salinity on cyanobacterial community composition along a transect from Fuliya spring into the water of Lake Kinneret, Israel
    Junier, Pilar 
    ;
    Kim, Ok-Sun
    ;
    Imhoff, Johannes F
    ;
    Witzel, Karl-Paul
    ;
    Hadas, Ora
    Cyanobacterial community composition was studied along a salinity gradient from the saline Spring Fuliya towards the water column of Lake Kinneret. The samples included a gradient of salinities ranging from 4270 mg Cl L–1 (Saline Spring) to 239 mg Cl L–1 (Lake Kinneret). Denaturing gradient gel electrophoresis (DGGE) and cloning of the 16 S rRNA gene, as well as cloning and sequencing of the psbA gene, were used to characterize cyanobacterial community composition. Despite the differences in salinity, similar cyanobacterial communities were observed in the lake and the saline spring, the only exception being the highest salinity sample (4270 mg Cl L–1). Both, DGGE patterns and results of the clone libraries revealed the dominance of cyanobacteria with colonial Gloeocapsa and unicellular Synechococcus as the closest known cultured relatives, independently of the salinity. These results suggest that cyanobacterial populations inhabiting this freshwater lake and its saline sources can adapt to a wide range of salinities.