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Neuhaus, Jean-Marc

Nom
Neuhaus, Jean-Marc
Affiliation principale
Fonction
Professeur ordinaire
Email
jean-marc.neuhaus@unine.ch
Identifiants
https://libra.unine.ch/handle/123456789/300

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  • Publication
    Accès libre
    ArabidopsisµA-adaptin interacts with the tyrosine motif of the vacuolar sorting receptor VSR-PS1
    (2004)
    Happel, Nicole
    ;
    Höning, Stefan
    ;
    Neuhaus, Jean-Marc 
    ;
    Paris, Nadine
    ;
    Robinson, David G.
    ;
    Holstein, Suzanne E. H.
    In receptor-mediated transport pathways in mammalian cells, clathrin-coated vesicle (CCV) µ-adaptins are the main binding partners for the tyrosine sorting/internalization motif (YXXØ). We have analyzed the function of the µA-adaptin, one of the five µ-adaptins from Arabidopsis thaliana, by pull-down assays and plasmon resonance measurements using its receptor-binding domain (RBD) fused to a histidine tag. We show that this adaptin is able to bind the consensus tyrosine motif YXXØ from the pea vacuolar sorting receptor (VSR)-PS1, as well as from the mammalian trans-Golgi network (TGN)38 protein. Moreover, the tyrosine residue was revealed to be crucial for binding of the complete cytoplasmic tail of VSR-PS1 to the plant µA-adaptin. The trans-Golgi localization of the µA-adaptin strongly suggests its involvement in Golgi- to vacuole-trafficking events.