Options
Filippidou, Sevasti
Nom
Filippidou, Sevasti
Affiliation principale
Identifiants
Résultat de la recherche
9 Résultats
Voici les éléments 1 - 9 sur 9
- PublicationAccès libreGenome Sequence of Anoxybacillus geothermalis Strain GSsed3, a Novel Thermophilic Endospore-Forming Species(2015)
; ;Jaussi, Marion ;Junier, Thomas; ; ; ;Vieth-Hillebrand, Andrea ;Vetter, Alexandra ;Regenspurg, Simona ;Johnson, Shannon ;McMurry, Kim ;Gleasner, Cheryl D. ;Lo, Chien-Chi ;Li, Po-E ;Vuyisich, Momchilo ;Chain, Patrick S.Anoxybacillus geothermalis strain GSsed3 is an endospore-forming thermophilic bacterium isolated from filter deposits in a geothermal site. This novel species has a larger genome size (7.2 Mb) than that of any other Anoxybacillus species, and it possesses genes that support its phenotypic metabolic characterization and suggest an intriguing link to metals. - PublicationAccès libreGenome Sequence of Aeribacillus pallidus Strain GS3372, an Endospore-Forming Bacterium Isolated in a Deep Geothermal Reservoir(2015)
; ;Jaussi, Marion ;Junier, Thomas; ; ;Regenspurg, Simona ;Li, Po-E ;Lo, Chien-Chi ;Johnson, Shannon ;McMurry, Kim ;Gleasner, Cheryl D. ;Vuyisich, Momchilo ;Chain, Patrick S.The genome of strain GS3372 is the first publicly available strain of Aeribacillus pallidus. This endospore-forming thermophilic strain was isolated from a deep geothermal reservoir. The availability of this genome can contribute to the clarification of the taxonomy of the closely related Anoxybacillus, Geobacillus, and Aeribacillus genera. - PublicationAccès libreGenome Sequence of Bacillus alveayuensis Strain 24KAM51, a Halotolerant Thermophile Isolated from a Hydrothermal Vent(2015)
; ; ;Junier, Thomas; ;Johnson, Shannon ;McMurry, Kim ;Gleasner, Cheryl D. ;Lo, Chien-Chi ;Li, Po-E ;Vuyisich, Momchilo ;Chain, Patrick S.Bacillus alveayuensis strain 24KAM51 was isolated from a marine hydrothermal vent in Milos, Greece. Its genome depicts interesting features of halotolerance and resistance to heavy metals. - PublicationAccès libreExploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium(2015)
; ; ; ;Wick, Lukas Y.; ;Kooli, Wafa M. ;Verrecchia, Eric P.Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. We present the validation and the results of the first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax soli, Olivibacter soli, Acinetobacter calcoaceticus, and several species of the genera Stenotrophomonas, Achromobacter and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications of the columns include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods. - PublicationAccès libreGenome Sequence of Kosakonia radicincitans Strain YD4, a Plant Growth-Promoting Rhizobacterium Isolated from Yerba Mate (Ilex paraguariensis St. Hill.)
;Bergottini, Veronica M; ; ;Johnson, Shannon ;Chain, Patrick S ;Otegui, Monica B ;Zapata, Pedro DKosakonia radicincitans strain YD4 is a rhizospheric isolate from yerba mate (Ilex paraguariensis St. Hill.) with plant growth-promoting effects on this crop. Genes involved in different plant growth-promoting activities are present in this genome, suggesting its potential as a bioinoculant for yerba mate. - PublicationAccès libreGains of Bacterial Flagellar Motility in a Fungal World
;Pion, Martin; ; ; ;Wick, Lukas Y; The maintenance of energetically costly flagella by bacteria in non-water-saturated media, such as soil, still presents an evolutionary conundrum. Potential explanations have focused on rare flooding events allowing dispersal. Such scenarios, however, overlook bacterial dispersal along mycelia as a possible transport mechanism in soils. The hypothesis tested in this study is that dispersal along fungal hyphae may lead to an increase in the fitness of flagellated bacteria and thus offer an alternative explanation for the maintenance of flagella even in unsaturated soils. Dispersal along fungal hyphae was shown for a diverse array of motile bacteria. To measure the fitness effect of dispersal, additional experiments were conducted in a model system mimicking limited dispersal, using Pseudomonas putida KT2440 and its nonflagellated (ΔfliM) isogenic mutant in the absence or presence of Morchella crassipes mycelia. In the absence of the fungus, flagellar motility was beneficial solely under conditions of water saturation allowing dispersal, while under conditions limiting dispersal, the nonflagellated mutant exhibited a higher level of fitness than the wild-type strain. In contrast, in the presence of a mycelial network under conditions limiting dispersal, the flagellated strain was able to disperse using the mycelial network and had a higher level of fitness than the mutant. On the basis of these results, we propose that the benefit of mycelium-associated dispersal helps explain the persistence of flagellar motility in non-water-saturated environments. - PublicationAccès libreUnder-detection of endospore-forming Firmicutes in metagenomic data
; ; ;Wunderlin, Tina ;Lo, Chien-Chi ;Li, Po-E ;Chain, Patrick SMicrobial diversity studies based on metagenomic sequencing have greatly enhanced our knowledge of the microbial world. However, one caveat is the fact that not all microorganisms are equally well detected, questioning the universality of this approach. Firmicutes are known to be a dominant bacterial group. Several Firmicutes species are endospore formers and this property makes them hardy in potentially harsh conditions, and thus likely to be present in a wide variety of environments, even as residents and not functional players. While metagenomic libraries can be expected to contain endospore formers, endospores are known to be resilient to many traditional methods of DNA isolation and thus potentially undetectable. In this study we evaluated the representation of endospore-forming Firmicutes in 73 published metagenomic datasets using two molecular markers unique to this bacterial group (spo0A and gpr). Both markers were notably absent in well-known habitats of Firmicutes such as soil, with spo0A found only in three mammalian gut microbiomes. A tailored DNA extraction method resulted in the detection of a large diversity of endospore-formers in amplicon sequencing of the 16S rRNA and spo0A genes. However, shotgun classification was still poor with only a minor fraction of the community assigned to Firmicutes. Thus, removing a specific bias in a molecular workflow improves detection in amplicon sequencing, but it was insufficient to overcome the limitations for detecting endospore-forming Firmicutes in whole-genome metagenomics. In conclusion, this study highlights the importance of understanding the specific methodological biases that can contribute to improve the universality of metagenomic approaches. - PublicationAccès libreGenome Sequence of Aeribacillus pallidus Strain GS3372, an Endospore-Forming Bacterium Isolated in a Deep Geothermal Reservoir
; ;Jaussi, Marion; ;Wunderlin, Tina; ;Regenspurg, Simona ;Li, Po-E ;Lo, Chien-Chi ;Johnson, Shannon ;McMurry, Kim ;Gleasner, Cheryl D ;Vuyisich, Momchilo ;Chain, Patrick SThe genome of strain GS3372 is the first publicly available strain of Aeribacillus pallidus. This endospore-forming thermophilic strain was isolated from a deep geothermal reservoir. The availability of this genome can contribute to the clarification of the taxonomy of the closely related Anoxybacillus, Geobacillus, and Aeribacillus genera. - PublicationAccès libreGenome Sequence of Bacillus alveayuensis Strain 24KAM51, a Halotolerant Thermophile Isolated from a Hydrothermal Vent
; ;Wunderlin, Tina; ; ;Johnson, Shannon ;McMurry, Kim ;Gleasner, Cheryl D ;Lo, Chien-Chi ;Li, Po-E ;Vuyisich, Momchilo ;Chain, Patrick SBacillus alveayuensis strain 24KAM51 was isolated from a marine hydrothermal vent in Milos, Greece. Its genome depicts inter-esting features of halotolerance and resistance to heavy metals.