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Gillet, François

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Gillet, François
Affiliation principale
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https://libra.unine.ch/handle/123456789/781

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  • Publication
    Accès libre
    How elevated pCO2 modifies total and metabolically active bacterial communities in the rhizosphere of two perennial grasses grown under field conditions
    (2006)
    Jossi, Maryline
    ;
    Fromin, Nathalie
    ;
    Tarnawski, Sonia
    ;
    Kohler, Florian
    ;
    Gillet, François 
    ;
    Aragno, Michel 
    ;
    Hamelin, Jérôme
    The response of total (DNA-based analysis) and active (RNA-based analysis) bacterial communities to a pCO2 increase under field conditions was assessed using two perennial grasses: the nitrophilic Lolium perenne and the oligonitrophilic Molinia coerulea. PCR- and reverse transcriptase-PCR denaturing gradient gel electrophoresis analysis of 16S rRNA genes generated contrasting profiles. The pCO2 increase influenced mainly the active and root-associated component of the bacterial community. Bacterial groups responsive to the pCO2 increase were identified by sequencing of corresponding denaturing gradient gel electrophoresis bands. About 50% of retrieved sequences were affiliated to Proteobacteria. Our data suggest that Actinobacteria in soil and Myxococcales (Deltaproteobacteria) in root are stimulated under elevated pCO2.