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Gern, Lise

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Gern, Lise
Affiliation principale
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Ancien.ne collaborateur.trice
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https://libra.unine.ch/handle/123456789/303

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  • Publication
    Métadonnées seulement
    Characterization of Borrelia lusitaniae isolates collected in Tunisia and Morocco
    (2005)
    Younsi, Hend
    ;
    Sarih, M'Hammed
    ;
    Jouda, Fatima
    ;
    Godfroid, Edmond
    ;
    Gern, Lise 
    ;
    Bouattour, Ali
    ;
    Baranton, Guy
    ;
    Postic, Danièle
    Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato and is infrequently isolated in Europe. In contrast, this species is by far the most predominant in North Africa and in Portugal. In this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from free-living Ixodes ricinus ticks collected in Tunisia and Morocco. We found a moderate diversity of the whole genome by using pulsed-field gel electrophoresis as well as in the ospA gene sequences, compared to a high level of strain homogeneity in the small noncoding ribosomal spacer. In contrast, a high diversity of this locus has been previously reported for Portuguese isolates. We hypothesize that B. lusitaniae strains isolated in North Africa constitute a clone of Portuguese origin.
  • Publication
    Métadonnées seulement
    First isolation of Borrelia burgdorferi sensu lato from Ixodes ricinus ticks in Morocco
    (2003)
    Sarih, M'Hammed
    ;
    Jouda, Fatima
    ;
    Gern, Lise 
    ;
    Postic, Danièle
    To determine the infection rate of Ixodes ricinus (I. ricinus) ticks with Borrelia burgdorferi sensu lato (B. burgdorferi sl) and to assess the frequency of the individual Borrelia species in this tick species, a total of 295 I. ricinus were collected in Taza region (Northeast of Morocco), from January to June 2002. The presence of B. burgdorferi A was determined by direct fluorescence antibody assay (DFA) and by PCR after culture. B. burgdorferi sl isolates were identified at the species level by restriction fragment length polymorphism. analysis of amplified products. The mean rate of I. ricinus infection with B. burgdorferi sl was 47.8%. Isolation attempts in BSK II medium resulted in 26 pure isolates. However, PCR performed on culture medium allowed to identify 82 Borrelia DNAs. B. lusitaniae has been identified from 76 out of 82 infected I. ricinus ticks (92.7%). Three ticks were infected by B. burgdorferi ss, and three other ticks were infected by B. garinii. This is the first report of the presence of B. burgdorferi A in Morocco and more specifically of B. burgdorferi ss in North Africa.