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Gern, Lise

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Gern, Lise
Affiliation principale
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Ancien.ne collaborateur.trice
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https://libra.unine.ch/handle/123456789/303

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  • Publication
    Métadonnées seulement
    Presence of potentially pathogenic Babesia sp for human in Ixodes ricinus in Switzerland
    (2006)
    Casati, Simona
    ;
    Sager, Heinz
    ;
    Gern, Lise 
    ;
    Piffaretti, Jean-Claude
    We have designed and performed a new PCR method based on the 18S rRNA in order to individuate the presence and the identity of Babesia parasites. Out of H 59 Ixodes ricinus (Acari: Ixodidae) ticks collected in four areas of Switzerland, nine were found to contain Babesia DNA. Sequencing of the short amplicon obtained (411 452 bp) allowed the identification of three human pathogenic species: Babesia microti, B. divergens, for the first time in Switzerland, Babesia sp. EU1. We also report coinfections with B. sp. EU1-Borrelia burgdoferi sensu stricto and Babesia sp. EU1-B. afzelii.
  • Publication
    Accès libre
    Diversity within Borrelia burgdorferi sensu lato genospecies in Switzerland by recA gene sequence
    (2004)
    Casati, Simona
    ;
    Bernasconi, Marco V.
    ;
    Gern, Lise 
    ;
    Piffaretti, Jean-Claude
    A total of 874 Ixodes ricinus ticks were collected in Switzerland to investigate the genetic diversity of the Borrelia population. We integrated to the RT-PCR method the DNA sequence analysis of a 162-bp fragment of the recA gene. Five genospecies were detected: Borrelia afzelii, Borrelia burgdorferi s.s., Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. A heterogeneous distribution was observed within the B. burgdorferi s.l. genospecies. The most prevalent and diverse genospecies found in Switzerland was Borrelia afzelii, which might suggest a rapid evolution of this genospecies.