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Gern, Lise

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Gern, Lise
Affiliation principale
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Ancien.ne collaborateur.trice
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https://libra.unine.ch/handle/123456789/303

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  • Publication
    Métadonnées seulement
    Detection and identification of Ehrlichia spp. in ticks collected in Tunisia and Morocco
    (2005)
    Sarih, M'Hammed
    ;
    M'Ghirbi, Youmna
    ;
    Bouattour, Ali
    ;
    Gern, Lise 
    ;
    Baranton, Guy
    ;
    Postic, Danièle
    A broad-range 16S rRNA gene PCR assay followed by partial sequencing of the 16S rRNA gene was used for the detection of members of the family Anaplasmataceae in ticks in North Africa. A total of 418 questing Ixodes ricinus ticks collected in Tunisia and Morocco, as well as 188 Rhipicephalus ticks from dogs and 52 Hyalomma ticks from bovines in Tunisia, were included in this study. Of 324 adult L ricinus ticks, 16.3% were positive for Ehrlichia spp., whereas only 3.4 and 2.8% of nymphs and larvae, respectively, were positive. A large heterogeneity was observed in the nucleotide sequences. Partial sequences identical to that of the agent of human granulocytic ehrlichiosis (HGE) were detected in L ricinus and Hyalomma detritum, whereas partial sequences identical to that of Anaplasma platys were detected in Rhipicephalus sanguineus. However, variants of Anaplasma, provisionally designated Anaplasma-like, were predominant in the L ricinus tick population in Maghreb. Otherwise, two variants of the genus Ehrlichia were detected in L ricinus and H. detritum. Surprisingly, a variant of Wolbachia pipientis was evidenced from L ricinus in Morocco. These results emphasized the potential risk of tick bites for human and animal populations in North Africa.
  • Publication
    Métadonnées seulement
    Characterization of Borrelia lusitaniae isolates collected in Tunisia and Morocco
    (2005)
    Younsi, Hend
    ;
    Sarih, M'Hammed
    ;
    Jouda, Fatima
    ;
    Godfroid, Edmond
    ;
    Gern, Lise 
    ;
    Bouattour, Ali
    ;
    Baranton, Guy
    ;
    Postic, Danièle
    Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato and is infrequently isolated in Europe. In contrast, this species is by far the most predominant in North Africa and in Portugal. In this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from free-living Ixodes ricinus ticks collected in Tunisia and Morocco. We found a moderate diversity of the whole genome by using pulsed-field gel electrophoresis as well as in the ospA gene sequences, compared to a high level of strain homogeneity in the small noncoding ribosomal spacer. In contrast, a high diversity of this locus has been previously reported for Portuguese isolates. We hypothesize that B. lusitaniae strains isolated in North Africa constitute a clone of Portuguese origin.