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Talking with the neighbours: Understanding spatial accommodation during plant development
Titre du projet
Talking with the neighbours: Understanding spatial accommodation during plant development
Description
Turgor-driven pressure of plant cells can be higher than that of a car tire. It puts tremendous forces onto cell walls and drives changes in cell shape. This has driven unique mechanisms to control organ formation in comparison to metazoans. The fascinating interplay between forces and local cellular reorganization is poorly understood. Growth of lateral roots is a prominent example of a developmental process in which mechanical forces are generated. Lateral roots grow from a single cell layer that resides deep within the primary root. On its way out, lateral roots grow through endodermal, cortical and epidermal cell layers. I recently demonstrated that endodermal cells actively accommodate lateral root formation. Responses include a dramatic volume loss and a controlled degradation of their lignin-based paracellular diffusion barrier. Interfering genetically with these accommodating responses in the endodermis completely blocks cell proliferation in the pericycle. The lateral root system
provides a unique opportunity to elucidate the molecular and cellular mechanisms underlying spatial accommodation in plant development. I will combine genetic screens, state-of-the-art transcript profiling and advanced microscopy to identify the molecular players and mechanisms regulating spatial accommodation. Beyond its importance for lateral root formation, this research can provide fundamental new insights into many other developmental processes that heavily depend on spatial accommodation by surrounding tissue. These include the growth of pollen tubes and infection threads, the development of sclerenchyma fibre cells or the intracellular accommodation of symbionts.
provides a unique opportunity to elucidate the molecular and cellular mechanisms underlying spatial accommodation in plant development. I will combine genetic screens, state-of-the-art transcript profiling and advanced microscopy to identify the molecular players and mechanisms regulating spatial accommodation. Beyond its importance for lateral root formation, this research can provide fundamental new insights into many other developmental processes that heavily depend on spatial accommodation by surrounding tissue. These include the growth of pollen tubes and infection threads, the development of sclerenchyma fibre cells or the intracellular accommodation of symbionts.
Chercheur principal
Statut
Completed
Date de début
1 Octobre 2015
Date de fin
30 Septembre 2021
Chercheurs
Nenadić, Milica
Thellmann, Martha
Stöckle, Dorothee
Shekhar, Vinay
Organisations
Identifiant interne
49137
identifiant
Mots-clés
5 Résultats
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- PublicationAccès libreGDSL-domain proteins have key roles in suberin polymerization and degradation(2021-3-8)
- PublicationRestriction temporaireTranslating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale(2020-5-1)
- PublicationAccès libreGeometric cues forecast the switch from two- to three-dimensional growth in Physcomitrella patens(2020-12-3)
; Willemsen, ViolaDuring land colonization, plants acquired a range of body plan adaptations, of which the innovation of three-dimensional (3D) tissues increased organismal complexity and reproductivity. In the moss, Physcomitrella patens, a 3D leafy gametophore originates from filamentous cells that grow in a two-dimensional (2D) plane through a series of asymmetric cell divisions. Asymmetric cell divisions that coincide with different cell division planes and growth directions enable the developmental switch from 2D to 3D, but insights into the underlying mechanisms coordinating this switch are still incomplete. Using 2D and 3D imaging and image segmentation, we characterized two geometric cues, the width of the initial cell and the angle of the transition division plane, which sufficiently distinguished a gametophore initial cell from a branch initial cell. These identified cues were further confirmed in gametophore formation mutants. The identification of a fluorescent marker allowed us to successfully predict the gametophore initial cell with > 90% accuracy before morphological changes, supporting our hypothesis that, before the transition division, parental cells of the gametophore initials possess different properties from those of the branch initials. Our results suggest that the cell fate decision of the initial cell is determined in the parental cell, before the transition division. - PublicationAccès libreCytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis(2019-7-18)
; Maizel, AlexisHow plant cells re-establish differential growth to initiate organs is poorly understood. Morphogenesis of lateral roots relies on the asymmetric cell division of initially symmetric founder cells. This division is preceded by the tightly controlled asymmetric radial expansion of these cells. The cellular mechanisms that license and ensure the coordination of these events are unknown. Here, we quantitatively analyze microtubule and F-actin dynamics during lateral root initiation. Using mutants and pharmacological and tissue-specific genetic perturbations, we show that dynamic reorganization of both microtubule and F-actin networks is necessary for the asymmetric expansion of the founder cells. This cytoskeleton remodeling intertwines with auxin signaling in the pericycle and endodermis in order for founder cells to acquire a basic polarity required for initiating lateral root development. Our results reveal the conservation of cell remodeling and polarization strategies between the Arabidopsis zygote and lateral root founder cells. We propose that coordinated, auxin-driven reorganization of the cytoskeleton licenses asymmetric cell growth and divisions during embryonic and post-embryonic organogenesis. - PublicationAccès librePluripotent Pericycle Cells Trigger Different Growth Outputs by Integrating Developmental Cues into Distinct Regulatory Modules(2020-9-10)
; Ragni, LauraDuring post-embryonic development, the pericycle specifies the stem cells that give rise to both lateral roots (LRs) and the periderm, a suberized barrier that protects the plant against biotic and abiotic stresses. Comparable auxin-mediated signaling hubs regulate meristem establishment in many developmental contexts; however, it is unknown how specific outputs are achieved. Using the Arabidopsis root as a model, we show that while LR formation is the main auxin-induced program after de-etiolation, plants with age become competent to form a periderm in response to auxin. The establishment of the vascular cambium acts as the developmental switch required to trigger auxin-mediated periderm initiation. Moreover, distinct auxin signaling components and targets control LR versus periderm formation. Among the periderm-specific-promoting transcription factors, WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNAT1/BREVIPEDICELLUS (BP) stand out as their specific overexpression in the periderm results in an increased number of periderm layers, a trait of agronomical importance in breeding programs targeting stress tolerance. These findings reveal that specificity in pericycle stem cell fate is achieved by the integration of developmental cues into distinct regulatory modules.