Chloroplast lipid droplet type II NAD(P)H quinone oxidoreductase is essential for prenylquinone metabolism and vitamin K(1) accumulation

Eugeni Piller, Lucia; Besagni, Céline; Ksas, Brigitte; Rumeau, Dominique; Bréhélin, Claire; Glauser, Gaétan; Kessler, Félix; Havaux, Michel

doi:10.1073/pnas.1104790108

Chloroplast lipid droplet type II NAD(P)H quinone oxidoreductase is essential for prenylquinone metabolism and vitamin K(1) accumulation

Author(s)

Eugeni Piller, Lucia

Faculté des sciences

Besagni, Céline

Ksas, Brigitte

Rumeau, Dominique

Bréhélin, Claire

Glauser, Gaétan

Kessler, Félix

Laboratoire de physiologie végétale

Havaux, Michel

Date issued

2011

In

Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences

Vol

108

No

34

From page

14354

To page

14359

Subjects

lipidomics alternative electron flow high light

Abstract

Lipid droplets are ubiquitous cellular structures in eukaryotes and are required for lipid metabolism. Little is currently known about plant lipid droplets other than oil bodies. Here, we define dual roles for chloroplast lipid droplets (plastoglobules) in energy and prenylquinone metabolism. The prenylquinones—plastoquinone, plastochromanol-8, phylloquinone (vitamin K(1)), and tocopherol (vitamin E)—are partly stored in plastoglobules. This work shows that NAD(P)H dehydrogenase C1 (NDC1) (At5g08740), a type II NAD(P)H quinone oxidoreductase, associates with plastoglobules. NDC1 reduces a plastoquinone analog in vitro and affects the overall redox state of the total plastoquinone pool in vivo by reducing the plastoquinone reservoir of plastoglobules. Finally, NDC1 is required for normal plastochromanol-8 accumulation and is essential for vitamin K(1) production.