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Antigenic conservation of an immunodominant invariable region of the VlsE lipoprotein among European pathogenic genospecies of Borrelia burgdorferi SL
Auteur(s)
Liang, Fang Ting
Aberer, Elisabeth
Cinco, Marina
Hu, Chang Min
Lobet, Yves
Ruscio, Maurizio
Voet, Pierre
Weynants, Vincent
Philipp, Mario T
Date de parution
2000
In
Journal of Infectious Diseases
Vol.
5
No
182
De la page
1455
A la page
1462
Mots-clés
Résumé
Lyme disease is caused by genetically divergent spirochetes, including 3 pathogenic genospecies: Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii. Serodiagnosis is complicated by this genetic diversity. A synthetic peptide (C-6), based on the 26-mer invariable region (IR6) of the variable surface antigen of B. burgdorferi (VlsE), was used as ELISA antigen, to test serum samples collected from mice experimentally infected with the 3 genospecies and from European patients with Lyme disease, Regardless of the infecting strains, mice produced a strong antibody response to C-6, which indicates that IR6 is antigenically conserved among the pathogenic genospecies. Twenty of 23 patients with culture-confirmed erythema migrans had a detectable antibody response to C-6, A sensitivity of 95.2% was achieved, with serum samples collected from patients with well-defined acrodermatitis chronica atrophicans, Fourteen of 20 patients with symptoms of late Lyme disease also had a positive anti-IR6 ELISA, Thus, it is possible that C-6 may be used to serodiagnose Lyme disease universally.
Identifiants
Type de publication
journal article
