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Combined arene ruthenium porphyrins as chemotherapeutics and photosensitizers for cancer therapy
Auteur(s)
Schmitt, Frédéric
Govindaswamy, Padavattan
Zava, Olivier
Juillerat-Jeanneret, Lucienne
Date de parution
2009
In
Journal of Biological Inorganic Chemistry, Springer, 2009/14/1/101-109
Résumé
Mononuclear 5-(4-pyridyl)-10,15,20-triphenylporphyrin and 5-(3-pyridyl)-10,15,20-triphenylporphyrin as well as tetranuclear 5,10,15,20-tetra(4-pyridyl)porphyrin (tetra-4-pp) and 5,10,15,20-tetra(3-pyridyl)porphyrin) (tetra-3-pp) arene ruthenium(II) derivatives (arene is C<sub>6</sub>H<sub>5</sub>Me or p-Pr<sup><i>i</i></sup>C<sub>6</sub>H<sub>4</sub>Me) were prepared and evaluated as potential dual photosensitizers and chemotherapeutics in human Me300 melanoma cells. In the absence of light, all tetranuclear complexes were cytotoxic (IC<sub>50</sub> ≤ 20 μM), while the mononuclear derivatives were not (IC<sub>50</sub> ≥ 100 μM). Kinetic studies of tritiated thymidine and tritiated leucine incorporations in cells exposed to a low concentration (5 μM) of tetranuclear p-cymene derivatives demonstrated a rapid inhibition of DNA synthesis, while protein synthesis was inhibited only later, suggesting arene ruthenium–DNA interactions as the initial cytotoxic process. All complexes exhibited phototoxicities toward melanoma cells when exposed to laser light of 652 nm. At low concentration (5 μM), LD<sub>50</sub> of the mononuclear derivatives was between 5 and 10 J/cm<sup>2</sup>, while for the tetranuclear derivatives LD<sub>50</sub> was approximately 2.5 J/cm<sup>2</sup> for the [Ru<sub>4</sub> (η<sup>6</sup>-arene)<sub>4</sub> (tetra-4-pp)Cl<sub>8</sub>] complexes and less than 0.5 J/cm<sup>2</sup> for the [Ru<sub>4</sub> (η<sup>6</sup>-arene)<sub>4</sub> (tetra-3-pp)Cl<sub>8</sub>] complexes. Examination of cells under a fluorescence microscope revealed the [Ru<sub>4</sub> (η<sup>6</sup>-arene)<sub>4</sub> (tetra-4-pp)Cl<sub>8</sub>] complexes as cytoplasmic aggregates, whereas the [Ru4(η<sup>6</sup>-arene)4(tetra-3-pp)Cl<sub>8</sub>] complexes were homogenously dispersed in the cytoplasm. Thus, these complexes present a dual synergistic effect with good properties of both the arene ruthenium chemotherapeutics and the porphyrin photosensitizer.
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Type de publication
journal article
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