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Guerin, Patrick

Nom
Guerin, Patrick
Affiliation principale
Site web
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Email
patrick.guerin@unine.ch
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https://libra.unine.ch/handle/123456789/310

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  • Publication
    MƩtadonnƩes seulement
    Plant Volatiles Enhance Behavioral Responses of Grapevine Moth Males, Lobesia botrana to Sex Pheromone
    (2012)
    von Arx, M.
    ;
    Schmidt-Busser, D.
    ;
    Guerin, Patrick 
    Plant volatiles play an important role in the lives of phytophagous insects, by guiding them to oviposition, feeding and mating sites. We tested the effects of different host-plant volatiles on attraction of Lobesia botrana males to the female-produced sex pheromone, in a wind tunnel. Addition of volatile emissions from grapevines or individual plant volatiles to pheromone increased the behavioral responses of L. botrana males over those to pheromone alone. At a low release rate (under-dosed) of pheromone, addition of (E)-beta-caryophyllene, (Z)-3-hexenyl acetate, 1-hexanol, or 1-octen-3-ol increased all behavioral responses, from activation to pheromone source contact, while addition of (E)-4,8-dimethyl-1,3,7-nonatriene, (E)-beta-farnesene, (Z)-3-hexenol, or methyl salicylate affected only the initial behavioral responses. Dose-response experiments suggested an optimal release ratio of 1: 1000 (sex pheromone: host plant volatile). Our results highlight the role of plant volatiles in the sensory ecology of L. botrana.
  • Publication
    MƩtadonnƩes seulement
    An in Vitro Assay for Testing Mosquito Repellents Employing a Warm Body and Carbon Dioxide as a Behavioral Activator
    (2010)
    Kroeber, Thomas 
    ;
    Kessler, SĆ©bastien 
    ;
    Frei, JƩrƓme
    ;
    Bourquin, Martine 
    ;
    Guerin, Patrick 
    We describe here an in vitro behavioral assay for testing mosquito repellents applied in a dose-based manner to a warm body (34 C) in test cages. The system was used to assess the sensitivity of 4-6-day-old Anopheles gambiae to the insect repellent diethyl methyl benzamide (deet). These tests were made in the absence and presence of additional carbon dioxide (CO(2)) applied as a pulse to activate mosquitoes in the cages. In the absence of the CO(2) pulse the mosquitoes hardly responded to the warm body. Increasing the CO(2) level in the cage by 1,000 parts per million caused a 25-fold increase in the number of landings by mosquitoes on the warm body in 2-min tests. This mosquito activation allowed the measurement of a significant reduction in the number of landings to bite on the warm body with increasing doses of deet (0.4 to 3.8 mu g/cm(2)). An asymptotic nonlinear model fitted to the repellency data in the presence of CO(2) allowed estimation of the effective dose of deet that reduced landings to bite by 50% (ED(50)) at 0.95 mu g/cm(2) (5 nmol/cm(2)) and the corresponding ED(95) at 4.12 mu g/cm(2) (21.5 nmol/cm(2)). This in vitro bioassay has the advantage of permitting a fast throughput of test products under standardized conditions and is suitable for screenings designed for the purpose of discovering lead products with as yet unknown human toxicological and dermatological profiles.
  • Publication
    MƩtadonnƩes seulement
    Oriented responses of grapevine moth larvae Lobesia botrana to volatiles from host plants and an artificial diet on a locomotion compensator
    (2009)
    Becher, P. G.
    ;
    Guerin, Patrick 
    Larvae of the grapevine moth Lobesia botrana (Lepidoptera: Tortricidae) are a major pest of vine, Vitis vinifera. As larvae have limited energy reserves and are in danger of desiccation and predation an efficient response to plant volatiles that would guide them to food and shelter could be expected. The responses of starved 2nd or 3rd instar larvae to volatile emissions from their artificial diet and to single host plant volatiles were recorded on a locomotion compensator. Test products were added to an air stream passing over the 30 cm diameter servosphere. The larvae showed non-directed walks of low rectitude in the air stream alone but changed to goal-oriented upwind displacement characterised by relatively straight tracks when the odour of the artificial diet and vapours of methyl salicylate, 1-hexanol, (Z)-3-hexen-1-ol, terpinen-4-ol, 1-octen-3-ol, (E)-4,8-dimethyl-1,3,7-nonatriene and (Z)-3-hexenyl acetate were added to the air stream. This chemoanemotactic targeted displacement illustrates appetence for certain volatile cues from food by starved Lobesia larvae. Analysis of the larval behaviour indicates dose dependent responses to some of the host plant volatiles tested with a response to methyl salicylate already visible at 1 ng, the lowest source dose tested. These behavioural responses show that Lobesia larvae can efficiently locate mixtures of volatile products released by food sources as well as single volatile constituents of their host plants. Such goal-oriented responses with shorter travel time and reduced energy loss are probably of importance for larval survival as it decreases the time they are exposed to biotic and abiotic hazards. (C) 2009 Elsevier Ltd. All rights reserved.
  • Publication
    MƩtadonnƩes seulement
    In vitro assays for repellents and deterrents for ticks: differing effects of products when tested with attractant or arrestment stimuli
    (2003)
    McMahon, Conor
    ;
    Kroeber, Thomas 
    ;
    Guerin, Patrick 
    Most in vivo and in vitro tests with repellents or deterrents against ticks have not considered which sensory channel is being targeted. We have recorded the responses of two hard tick species (Acari: Ixodidae) in vitro to determine if such products can disrupt the perception of an attractant in a repellent assay or the perception of an arrestment stimulus in a deterrent assay. Ethyl butylacetylaminopropionate (EBAAP), N,N-diethyl-methyl-benzamide (deet), permethrin and indalone were chosen to test their capacity to inhibit the attraction of Amblyomma variegatum Fabricius to its aggregation-attachment pheromone. Vapours of each test product plus those from a synthetic blend of the pheromone were delivered to the walking tick in an air stream on a locomotion compensator. Neither EBAAP, deet, permethrin nor indalone could inhibit attraction of A. variegatum even when each of the test products was delivered at 10(6) times the pheromone. Indalone did decrease the attraction of A. variegatum to the pheromone and induced repulsion of A. variegatum when presented on its own in the air stream. The effect of permethrin, a sodium channel blocker, was also tested in a deterrent assay measuring the arrestment of Ixodes ricinus (L.) adults on its own faeces and faecal constituents. Permethrin deterred arrestment at doses of 670 fg/cm(2) to 67 ng/cm(2), i.e. at levels five times lower than the dose of chemostimuli present in the arrestment stimulus. This sensitivity to permethrin suggests that it acts via the contact chemoreception channel.
  • Publication
    AccĆØs libre
    In vitro assays for repellents and deterrents for ticks: differing effects of products when tested with attractant or arrestment stimuli
    (2003)
    McMahon, Conor
    ;
    Krƶber, Thomas
    ;
    Guerin, Patrick 
    Most in vivo and in vitro tests with repellents or deterrents against ticks have not considered which sensory channel is being targeted. We have recorded the responses of two hard tick species (Acari: Ixodidae) in vitro to determine if such products can disrupt the perception of an attractant in a repellent assay or the perception of an arrestment stimulus in a deterrent assay. Ethyl butylacetylaminopropionate (EBAAP), N,N-diethyl-methyl-benzamide (deet), permethrin and indalone were chosen to test their capacity to inhibit the attraction of Amblyomma variegatum Fabricius to its aggregation-attachment pheromone. Vapours of each test product plus those from a synthetic blend of the pheromone were delivered to the walking tick in an air stream on a locomotion compensator. Neither EBAAP, deet, permethrin nor indalone could inhibit attraction of A. variegatum even when each of the test products was delivered at 106 times the pheromone. Indalone did decrease the attraction of A. variegatum to the pheromone and induced repulsion of A. variegatum when presented on its own in the air stream. The effect of permethrin, a sodium channel blocker, was also tested in a deterrent assay measuring the arrestment of Ixodes ricinus (L.) adults on its own faeces and faecal constituents. Permethrin deterred arrestment at doses of 670 fg/cm2 to 67 ng/cm2, i.e. at levels five times lower than the dose of chemostimuli present in the arrestment stimulus. This sensitivity to permethrin suggests that it acts via the contact chemoreception channel.
  • Publication
    AccĆØs libre
    Identification of oviposition attractants for the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) in volatiles of faeces from vertebrates
    (1995)
    Dougherty, M. J.
    ;
    Guerin, Patrick 
    ;
    Ward, R. D.
    Extracts of volatiles from rabbit and chicken faeces preferentially attracted gravid sandflies, Lutzomyia longipalpis (Lutz and Neiva), in an oviposition bioassay. In electrophysiology experiments, the same extracts selectively stimulated two olfactory cells while inhibiting another in ascoid sensilla on the antennae of these flies. Analysis of faeces volatiles by gas chromatography linked to ascoid sensillum recording revealed two early eluting electrophysiologically active components of rabbit faeces. These active compounds were identified in both rabbit and chicken faeces volatile extracts by gas chromatography -mass spectrometry as hexanal and 2-methyl-2-butanol. Hexanal stimulated one cell type and inhibited another, whereas 2-methyl-2-butanol stimulated a third cell type. A 1:l mixture of synthetic hexanal and 2-methyl-2-butanol elicited the same targeted oviposition response from gravid females on the treatment septum of the bioassay as did the total volatile extract of rabbit or chicken faeces.
    The monoterpenes Ī±(+)-pinene (plus some optical and positional isomers) and a-terpinene activated a separate cell type, whereas benzaldehyde stimulated the same receptor as hexanal, but with a higher threshold. Furthermore, an olfactory cell selectively tuned to the perception of the male sex pheromone of this species was also found in the ascoid sensillum.