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Lara, Enrique

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Lara, Enrique
Affiliation principale
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https://libra.unine.ch/handle/123456789/360

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  • Publication
    Accès libre
    Cultivation-independent analysis reveals a shift in ciliate 18S rRNA gene diversity in a polycyclic aromatic hydrocarbon-polluted soil
    (2007)
    Lara, Enrique 
    ;
    Berney, Cédric
    ;
    Harms, Hauke
    ;
    Chatzinotas, Antonis
    Using cultivation-independent methods the ciliate communities of a clay-rich soil with a 90-year record of pollution by polycyclic aromatic hydrocarbons (PAH) (4.5 g kg−1 PAH) were compared with that of a nonpolluted soil collected in its vicinity and with similar properties. A ciliate-specific set of 18S rRNA gene targeting primers was designed and used to amplify DNA extracted from both soils (surface and 20 cm depth). Four clone libraries were generated with PCR products that covered an 18S rRNA gene fragment of up to 670 bp. Comparative sequence analysis of representative clones proved that the primer set was highly specific for ciliates. Calculation of similarity indices based on operational taxonomic units after amplified ribosomal DNA restriction analysis of the clones showed that the community from the nonpolluted surface soil was highly dissimilar to the other communities. The presence of several taxa, namely sequences affiliated to the orders Phyllopharyngia, Haptoria, Nassophorea, Peniculida and Scuticociliatia in samples from nonpolluted soil, points to the existence of various trophic functional groups. In contrast, the 18S rRNA gene diversity was much lower in the clone libraries from the polluted soil. More than 90% of these sequences belonged to the class Colpodea, a well-known clade of mainly bacterivorous and r-selected species, thus potentially also indicating a lower functional diversity.
  • Publication
    Accès libre
    The Revised Classification of Eukaryotes
    Adl, Sina M.
    ;
    Simpson, Alastair G. B.
    ;
    Lane, Christopher E.
    ;
    Lukeš, Julius
    ;
    Bass, David
    ;
    Bowser, Samuel S.
    ;
    Brown, Matthew W.
    ;
    Burki, Fabien
    ;
    Dunthorn, Micah
    ;
    Hampl, Vladimir
    ;
    Heiss, Aaron
    ;
    Hoppenrath, Mona
    ;
    Lara, Enrique 
    ;
    le Gall, Line
    ;
    Lynn, Denis H.
    ;
    McManus, Hilary
    ;
    Mitchell, Edward 
    ;
    Mozley-Stanridge, Sharon E.
    ;
    Parfrey, Laura W.
    ;
    Pawlowski, Jan
    ;
    Rueckert, Sonja
    ;
    Shadwick, Laura
    ;
    Schoch, Conrad L.
    ;
    Smirnov, Alexey
    ;
    Spiegel, Frederick W.
    This revision of the classification of eukaryotes, which updates that of Adl et al. [J. Eukaryot. Microbiol. 52 (2005) 399], retains an emphasis on the protists and incorporates changes since 2005 that have resolved nodes and branches in phylogenetic trees. Whereas the previous revision was successful in re-introducing name stability to the classification, this revision provides a classification for lineages that were then still unresolved. The supergroups have withstood phylogenetic hypothesis testing with some modifications, but despite some progress, problematic nodes at the base of the eukaryotic tree still remain to be statistically resolved. Looking forward, subsequent transformations to our understanding of the diversity of life will be from the discovery of novel lineages in previously under-sampled areas and from environmental genomic information.