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Stutz, Erhard

Nom
Stutz, Erhard
Affiliation principale
Fonction
Professeur.e émérite
Identifiants
https://libra.unine.ch/handle/123456789/32192

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  • Publication
    Accès libre
    Structure of the Euglena gracilis chloroplast gene (psbA) coding for the 32-kDa protein of Photosystem II
    (1984)
    Keller, Mario
    ;
    Stutz, Erhard 
    An EcoRI fragment from Euglena gracilis chloroplast DNA, called Eco.I (4.9 kbp), contains the gene for the ‘32-kDa’ thylakoid membrane protein of Photosystem II (psb A gene), the tRNA2Leu gene and additional sequences which possibly code for two proteins of unknown function. The transcription polarity is the same for all these coding sequences. The psb A gene is split: 4 introns (size range, 433–616 bp) separate 5 exons (size range, 39–579 bp) which code for a protein of Mr 38380 (345 amino acids). The Euglena protein is about 87% homologous with the higher plant counterparts but it contains 5 lysine residues.
  • Publication
    Accès libre
    Analysis of the Euglena gracilis chloroplast genome: Fragment Eco-I encodes the gene for the Mr 32 000–33 000 thylakoid protein of photosystem II reaction center
    (1982)
    Keller, Mario
    ;
    Stutz, Erhard 
    The DNA fragment Eco-I (4700 kilobasepairs) from the circular chloroplast genome of Euglena gracilis is shown to be transcribed in etioplasts at all stages of light-induced plastid development and in fully differentiated chloroplasts. Major stable transcription products are mRNAs of 14S and 17S. Using a rabbit reticulocyte lysate translation system we can show that the fragment Eco-I selects a mRNA which directs the synthesis of a Mr 32 000–33 000 polypeptide. Eco-I also hybridizes with a 330 basepair DNA probe cut from within the spinach chloroplast gene encoding the Mr 32 000 thylakoid membrane protein of the photosystem II reaction center. We conclude that the fragment Eco-I carries the corresponding Euglena gracilis chloroplast gene.