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Mitchell, Edward
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Mitchell, Edward
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edward.mitchell@unine.ch
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Voici les éléments 1 - 9 sur 9
- PublicationMétadonnées seulementThe Revised Classification of Eukaryotes (vol 59, pg 429, 2012)(2013)
;Adl, Sina M. ;Simpson, Alastair G. B. ;Lane, Christopher E. ;Lukes, Julius ;Bass, David ;Bowser, Samuel S. ;Brown, Matthew W. ;Burki, Fabien ;Dunthorn, Micah ;Hampl, Vladimir ;Heiss, Aaron ;Hoppenrath, M.; ;le Gall, Line ;Lynn, Denis H. ;McManus, Hilary; ;Mozley-Stanridge, Sharon E. ;Parfrey, Laura Wegener ;Pawlowski, Jan ;Rueckert, Sonja ;Shadwick, Laura ;Schoch, Conrad L. ;Smirnov, AlexeySpiegel, Frederick W. - PublicationMétadonnées seulementCBOL Protist Working Group: Barcoding Eukaryotic Richness beyond the Animal, Plant, and Fungal Kingdoms(2012)
;Pawlowski, Jan ;Audic, Stephane ;Adl, Sina M. ;Bass, David; ;Berney, Cedric ;Bowser, Samuel S. ;Cepicka, Ivan ;Decelle, Johan ;Dunthorn, Micah ;Fiore-Donno, Anna Maria ;Gile, Gillian H. ;Holzmann, Maria ;Jahn, Regine ;Jirku, Miloslav ;Keeling, Patrick J. ;Kostka, Martin ;Kudryavtsev, Alexander; ;Lukes, Julius ;Mann, David G.; ;Nitsche, Frank ;Romeralo, Maria ;Saunders, Gary W. ;Simpson, Alastair G. B. ;Smirnov, Alexey V. ;Spouge, John L. ;Stern, Rowena F. ;Stoeck, Thorsten ;Zimmermann, Jonas ;Schindel, Davidde Vargas, Colomban - PublicationMétadonnées seulementThe Revised Classification of Eukaryotes(2012)
;Adl, Sina M. ;Simpson, Alastair G. B. ;Lane, Christopher E. ;Lukes, Julius ;Bass, David ;Bowser, Samuel S. ;Brown, Matthew W. ;Burki, Fabien ;Dunthorn, Micah ;Hampl, Vladimir ;Heiss, Aaron ;Hoppenrath, Mona; ;le Gall, Line ;Lynn, Denis H. ;McManus, Hilary; ;Mozley-Stanridge, Sharon E. ;Parfrey, Laura Wegener ;Pawlowski, Jan ;Rueckert, Sonja ;Shadwick, Laura ;Schoch, Conrad L. ;Smirnov, AlexeySpiegel, Frederick W.This revision of the classification of eukaryotes, which updates that of Adl etal. [J. Eukaryot. Microbiol. 52 (2005) 399], retains an emphasis on the protists and incorporates changes since 2005 that have resolved nodes and branches in phylogenetic trees. Whereas the previous revision was successful in re-introducing name stability to the classification, this revision provides a classification for lineages that were then still unresolved. The supergroups have withstood phylogenetic hypothesis testing with some modifications, but despite some progress, problematic nodes at the base of the eukaryotic tree still remain to be statistically resolved. Looking forward, subsequent transformations to our understanding of the diversity of life will be from the discovery of novel lineages in previously under-sampled areas and from environmental genomic information. - PublicationAccès libreComparing Potential COI and SSU rDNA Barcodes for Assessing the Diversity and Phylogenetic Relationships of Cyphoderiid Testate Amoebae (Rhizaria: Euglyphida)(2011)
;Heger, Thierry J. ;Pawlowski, Jan; ;Leander, Brian S. ;Todorov, Milcho ;Golemansky, VassilThe mitochondrial Cytochrome Oxidase Subunit 1 gene (COI) has been promoted as an ideal “DNA barcode” for animal species and other groups of eukaryotes. However, the utility of the COI marker for species level discrimination and for phylogenetic analyses has yet to be tested within the Rhizaria. Accordingly, we analysed mitochondrial COI gene sequences and nuclear small subunit rDNA (SSU) sequences from several morphospecies of euglyphid testate amoebae (Cercozoa, Rhizaria) in order to evaluate the utility of these DNA markers for species discrimination and phylogenetic reconstructions. Sequences were obtained from eleven populations belonging to six Cyphoderia morphospecies that were isolated from field samples in North America and Europe. Mean inter-population COI sequence dissimilarities were on average 2.9 times greater than in the SSU, while the intra-population sequence dissimilarities were higher in the SSU (0-0.95%) than in the COI (0%); this suggests that the COI fragment is valuable for discriminating Cyphoderiidae isolates. Our study also demonstrated that COI sequences are useful for inferring phylogenetic relationships among Cyphoderiidae isolates. COI and SSU tree topologies were very similar even though the COI fragment used in these analyses (500 bp) was much shorter than the SSU sequences (1600 bp). Altogether, these results demonstrate the utility of the COI as a potential taxonomic DNA barcode for assessing cyphoderiid species diversity and for inferring phylogenetic relationships within the group. - PublicationAccès libreMolecular phylogeny of euglyphid testate amoebae (Cercozoa: Euglyphida) suggests transitions between marine supralittoral and freshwater/terrestrial environments are infrequent(2010)
;Heger, Thierry J.; ;Todorov, Milcho ;Golemansky, Vassil; ;Leander, Brian S.Pawlowski, JanMarine and freshwater ecosystems are fundamentally different regarding many biotic and abiotic factors. The physiological adaptations required for an organism to pass the salinity barrier are considerable. Many eukaryotic lineages are restricted to either freshwater or marine environments. Molecular phylogenetic analyses generally demonstrate that freshwater species and marine species segregate into different sub-clades, indicating that transitions between these two environments occur only rarely in the course of evolution. It is, however, unclear if the transitions between freshwater and environments characterized by highly variable salinities, such as the marine supralittoral zone, are also infrequent. Here, we use testate amoebae within the Euglyphida to assess the phylogenetic interrelationships between marine supralittoral and freshwater taxa. Euglyphid testate amoebae are mainly present in freshwater habitats but also occur in marine supralittoral environments. Accordingly, we generated and analyzed partial SSU rRNA gene sequences from 49 new marine/supralittoral and freshwater Cyphoderiidae sequences, 20 sequences of the Paulinellidae, Trinematidae, Assulinidae, and Euglyphidae families as well as 21 GenBank sequences of unidentified taxa derived from environmental PCR surveys. Both the molecular and morphological data suggest that the diversity of Cyphoderiidae is strongly underestimated. The results of our phylogenetic analyses demonstrated that marine supralittoral and freshwater euglyphid testate amoeba species are segregated into distinct sub-clades, suggesting that transitions between these two habitats occurred only infrequently. - PublicationMétadonnées seulementThe testate lobose amoebae (order Arcellinida Kent, 1880) finally find their home within Amoebozoa(2005)
;Nikolaev, Sergey I; ;Petrov, Nikolay B ;Berney, Cédric ;Fahrni, JoséPawlowski, JanTestate lobose amoebae (order Arcellinida Kent, 1880) are common in all aquatic and terrestrial habitats, yet they are one of the last higher taxa of unicellular eukaryotes that has not found its place in the tree of life. The morphological approach did not allow to ascertain the evolutionary origin of the group or to prove its monophyly. To solve these challenging problems, we analyzed partial small-subunit ribosomal RNA (SSU rRNA) genes of seven testate lobose amoebae from two out of the three suborders and seven out of the 13 families belonging to the Arcellinida. Our data support the monophyly of the order and clearly establish its position among Amoebozoa, as a sister-group to the clade comprising families Amoebidae and Hartmannellidae. Complete SSU rRNA gene sequences from two species and a partial actin sequence from one species confirm this position. Our phylogenetic analyses including representatives of all sequenced lineages of lobose amoebae suggest that a rigid test appeared only once during the evolution of the Amoebozoa, and allow reinterpretation of some morphological characters used in the systematics of Arcellinida. (c) 2005 Published by Elsevier GmbH. - PublicationAccès libreThe Testate Lobose Amoebae (Order Arcellinida Kent, 1880) Finally Find their Home within Amoebozoa(2005)
;Nikolaev, Sergey I.; ;Petrov, Nikolay B. ;Berney, Cédric ;Fahrni, JoséPawlowski, JanTestate lobose amoebae (order Arcellinida Kent, 1880) are common in all aquatic and terrestrial habitats, yet they are one of the last higher taxa of unicellular eukaryotes that has not found its place in the tree of life. The morphological approach did not allow to ascertain the evolutionary origin of the group or to prove its monophyly. To solve these challenging problems, we analyzed partial small-subunit ribosomal RNA (SSU rRNA) genes of seven testate lobose amoebae from two out of the three suborders and seven out of the 13 families belonging to the Arcellinida. Our data support the monophyly of the order and clearly establish its position among Amoebozoa, as a sister-group to the clade comprising families Amoebidae and Hartmannellidae. Complete SSU rRNA gene sequences from two species and a partial actin sequence from one species confirm this position. Our phylogenetic analyses including representatives of all sequenced lineages of lobose amoebae suggest that a rigid test appeared only once during the evolution of the Amoebozoa, and allow reinterpretation of some morphological characters used in the systematics of Arcellinida. - PublicationAccès libreThe Revised Classification of Eukaryotes
;Adl, Sina M. ;Simpson, Alastair G. B. ;Lane, Christopher E. ;Lukeš, Julius ;Bass, David ;Bowser, Samuel S. ;Brown, Matthew W. ;Burki, Fabien ;Dunthorn, Micah ;Hampl, Vladimir ;Heiss, Aaron ;Hoppenrath, Mona; ;le Gall, Line ;Lynn, Denis H. ;McManus, Hilary; ;Mozley-Stanridge, Sharon E. ;Parfrey, Laura W. ;Pawlowski, Jan ;Rueckert, Sonja ;Shadwick, Laura ;Schoch, Conrad L. ;Smirnov, AlexeySpiegel, Frederick W.This revision of the classification of eukaryotes, which updates that of Adl et al. [J. Eukaryot. Microbiol. 52 (2005) 399], retains an emphasis on the protists and incorporates changes since 2005 that have resolved nodes and branches in phylogenetic trees. Whereas the previous revision was successful in re-introducing name stability to the classification, this revision provides a classification for lineages that were then still unresolved. The supergroups have withstood phylogenetic hypothesis testing with some modifications, but despite some progress, problematic nodes at the base of the eukaryotic tree still remain to be statistically resolved. Looking forward, subsequent transformations to our understanding of the diversity of life will be from the discovery of novel lineages in previously under-sampled areas and from environmental genomic information. - PublicationAccès libreCBOL Protist Working Group: Barcoding Eukaryotic Richness beyond the Animal, Plant, and Fungal Kingdoms
;Pawlowski, Jan ;Audic, Stéphane ;Adl, Sina ;Bass, David; ;Berney, Cédric ;Bowser, Samuel S. ;Cepicka, Ivan ;Decelle, Johan ;Dunthorn, Micah ;Fiore-Donno, Anna Maria ;Gile, Gillian H. ;Holzmann, Maria ;Jahn, Regine ;Jirků, Miloslav ;Keeling, Patrick J. ;Kostka, Martin ;Kudryavtsev, Alexander; ;Lukeš, Julius ;Mann, David G.; ;Nitsche, Frank ;Romeralo, Maria ;Saunders, Gary W. ;Simpson, Alastair G. B. ;Smirnov, Alexey V. ;Spouge, John L. ;Stern,Rowena F. ;Stoeck, Thorsten ;Zimmermann, Jonas ;Schindel, Davidde Vargas, Colomban