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Isolation and characterization of polymorphic microsatellite loci in Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae)

2003, Alvarez, Nadir, Aebi, Alexandre, Risterucci, A.-M., Hossaert-McKey, Martine, Benrey, Betty

Five microsatellite loci were isolated from the bruchid Acanthoscelides obvelatus Bridwell (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from two to 15. We found high levels of within-population variation at most loci, with heterozygosity ranging from 0.182 to 0.900. Cross-species amplification of these loci was tested in two other species of the genus Acanthoscelides, A. obtectus Say and A. argillaceus Sharp.

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Horismenus species (Hymenoptera: Eulophidae) in a bruchid beetle parasitoid guild, including the description of a new species

, Hansson, Christer, Aebi, Alexandre, Benrey, Betty

Four species of Horismenus (Hymenoptera: Eulophidae) associated with Acanthoscelides spp.(Coleoptera: Bruchidae), three of which are also associated with Phaseolus spp., are treated. One of the species, H. butcheri, is described as new and the remaining three species are redescribed. Allfour species are diagnosed in a key. A lectotype is designated for Holcopelte productus Ashmead.

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Isolation and characterization of polymorphic microsatellite markers in Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae)

, Aebi, Alexandre, Shani, Tal, Butcher, R.D.J, Alvarez, N, Risterucci, A.M, Benrey, Betty

Bruchid beetles of the genus Zabrotes (Coleoptera: Bruchidae) are important worldwide pests of legume seeds. Samples of Zabrotes subfasciatus and Z. sylvestris were collected from seeds of two Phaseolus species throughout Mexico to assess the role of host plant variation and plant domestication on the evolution of host use in this species. For this purpose six polymorphic microsatellite loci were isolated and characterized for the bruchid Z. subfasciatus. Cross-species amplification tests were performed on Z. sylvestris and revealed that three loci amplified successfully and were polymorphic in this closely related species.