Résultat de la recherche
Microsatellite markers in a complex of Horismenus sp. (Hymenoptera: Eulophidae), parasitoids of bruchid beetles
Parasitoids of the genus Horismenus (Hymenoptera: Eulophidae) are the main natural enemies of bruchid beetles that feed on several species of Phaseolus beans. Samples of Horismenus depressus, H. missouriensis and H. butcheri were collected from seeds of three Phaseolus species throughout Mexico to examine the impact of plant variability on the genetic structure of parasitoid populations. For this purpose, six microsatellite loci were isolated and characterized. These loci are of great interest in understanding the taxonomy of the genus Horismenus, the most important Eulophid genus in the Neotropics.
Isolation and characterization of polymorphic microsatellite markers in Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae)
Bruchid beetles of the genus Zabrotes (Coleoptera: Bruchidae) are important worldwide pests of legume seeds. Samples of Zabrotes subfasciatus and Z. sylvestris were collected from seeds of two Phaseolus species throughout Mexico to assess the role of host plant variation and plant domestication on the evolution of host use in this species. For this purpose six polymorphic microsatellite loci were isolated and characterized for the bruchid Z. subfasciatus. Cross-species amplification tests were performed on Z. sylvestris and revealed that three loci amplified successfully and were polymorphic in this closely related species.