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Neier, Reinhard

Nom
Neier, Reinhard
Affiliation principale
Fonction
Professeure ordinaire
Email
Reinhard.Neier@unine.ch
Identifiants
https://libra.unine.ch/handle/123456789/383
_
0000-0003-0890-1797

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  • Publication
    Métadonnées seulement
    Glycoside Esters of 5-Aminolevulinic Acid for Photodynamic Therapy of Cancer
    (2008)
    Vallinayagam, Ramakrishnan
    ;
    Schmitt, Frederic
    ;
    Barge, Jerome
    ;
    Wagnieres, Georges
    ;
    Wenger, Virginie
    ;
    Neier, Reinhard 
    ;
    Juillerat-Jeanneret, Lucienne
    Aliph. and ethylene glycol esters of 5-aminolevulinic acid (ALA) are very efficient precursors of the photosensitizer protoporphyrin IX (PpIX) for photodynamic therapy; however, they diffuse passively across the cell membrane and thus lack cell selectivity. We evaluated whether ?-glucose, ?-mannose, or ?-galactose esters of ALA would present improved properties as precursors of PpIX. Esterification was performed either at the position O-1 or O-6 of the sugars with or without an ethylene glycol linker, and these glycoside esters of ALA were evaluated in human cells. The results demonstrated that glycoside esters of ALA are efficient precursors of PpIX in human cancer and angiogenic endothelial cells, comparable to free ALA, but not in normal human fibroblasts. PpIX prodn. was confirmed by fluorescence microscopy and photodynamic treatment of cells. The O-1 or O-6 positions of functionalization and the nature of the sugar moiety did not influence PpIX prodn. The presence of the ethylene glycol linker generally resulted in decreased PpIX prodn. The uptake of these glycoside esters of ALA by cells was not decreased in the presence of high concns. of the related sugars. Inhibitors of ?-glucosidases or ?-mannosidases did not decrease PpIX prodn. These results suggest the involvement of active non-glycoside-specific membrane transporter(s) for uptake and of esterases rather than glycosidases in the release of ALA from the glycoside esters of ALA. [on SciFinder(R)]
  • Publication
    Métadonnées seulement
    Novel bioconjugates of aminolevulinic acid with nucleosides
    (2008)
    Gurba, Patrice
    ;
    Vallinayagam, Ramakrishnan
    ;
    Schmitt, Frederic
    ;
    Furrer, Julien
    ;
    Juillerat-Jeanneret, Lucienne
    ;
    Neier, Reinhard 
    Esters and amino acid derivs. of 5-aminolevulinic acid (ALA) are efficient prodrugs for the prodn. of protoporphyrin IX (PpIX), which has been used in photodynamic cancer therapy (PDT). The synthesis of novel bioconjugates, e.g. I·CF3CO2H, combining ALA with adenosine and thymidine is reported. The novel bioconjugates have been made using a robust methodol. The new class of prodrugs contains one, two, or three ALA per mol. Preliminary cell tests in human cancer cell lines indicate that the thymidine conjugate of ALA is an efficient prodrug for PDT. [on SciFinder(R)]