Filippidou, Sevasti

2015, Filippidou, Sevasti, Jaussi, Marion, Junier, Thomas, Wunderlin, Tina, Jeanneret, Nicole, Regenspurg, Simona, Li, Po-E, Lo, Chien-Chi, Johnson, Shannon, McMurry, Kim, Gleasner, Cheryl D., Vuyisich, Momchilo, Chain, Patrick S., Junier, Pilar

The genome of strain GS3372 is the first publicly available strain of Aeribacillus pallidus. This endospore-forming thermophilic strain was isolated from a deep geothermal reservoir. The availability of this genome can contribute to the clarification of the taxonomy of the closely related Anoxybacillus, Geobacillus, and Aeribacillus genera.

, Filippidou, Sevasti, Jaussi, Marion, Junier, Thomas, Wunderlin, Tina, Jeanneret, Nicole, Regenspurg, Simona, Li, Po-E, Lo, Chien-Chi, Johnson, Shannon, McMurry, Kim, Gleasner, Cheryl D, Vuyisich, Momchilo, Chain, Patrick S, Junier, Pilar

The genome of strain GS3372 is the first publicly available strain of Aeribacillus pallidus. This endospore-forming thermophilic strain was isolated from a deep geothermal reservoir. The availability of this genome can contribute to the clarification of the taxonomy of the closely related Anoxybacillus, Geobacillus, and Aeribacillus genera.

2015, Filippidou, Sevasti, Wunderlin, Tina, Junier, Thomas, Jeanneret, Nicole, Johnson, Shannon, McMurry, Kim, Gleasner, Cheryl D., Lo, Chien-Chi, Li, Po-E, Vuyisich, Momchilo, Chain, Patrick S., Junier, Pilar

Bacillus alveayuensis strain 24KAM51 was isolated from a marine hydrothermal vent in Milos, Greece. Its genome depicts interesting features of halotolerance and resistance to heavy metals.

, Filippidou, Sevasti, Junier, Thomas, Wunderlin, Tina, Lo, Chien-Chi, Li, Po-E, Chain, Patrick S, Junier, Pilar

Microbial diversity studies based on metagenomic sequencing have greatly enhanced our knowledge of the microbial world. However, one caveat is the fact that not all microorganisms are equally well detected, questioning the universality of this approach. Firmicutes are known to be a dominant bacterial group. Several Firmicutes species are endospore formers and this property makes them hardy in potentially harsh conditions, and thus likely to be present in a wide variety of environments, even as residents and not functional players. While metagenomic libraries can be expected to contain endospore formers, endospores are known to be resilient to many traditional methods of DNA isolation and thus potentially undetectable. In this study we evaluated the representation of endospore-forming Firmicutes in 73 published metagenomic datasets using two molecular markers unique to this bacterial group (spo0A and gpr). Both markers were notably absent in well-known habitats of Firmicutes such as soil, with spo0A found only in three mammalian gut microbiomes. A tailored DNA extraction method resulted in the detection of a large diversity of endospore-formers in amplicon sequencing of the 16S rRNA and spo0A genes. However, shotgun classification was still poor with only a minor fraction of the community assigned to Firmicutes. Thus, removing a specific bias in a molecular workflow improves detection in amplicon sequencing, but it was insufficient to overcome the limitations for detecting endospore-forming Firmicutes in whole-genome metagenomics. In conclusion, this study highlights the importance of understanding the specific methodological biases that can contribute to improve the universality of metagenomic approaches.

2015, Filippidou, Sevasti, Jaussi, Marion, Junier, Thomas, Wunderlin, Tina, Roussel-Delif, Ludovic, Jeanneret, Nicole, Vieth-Hillebrand, Andrea, Vetter, Alexandra, Regenspurg, Simona, Johnson, Shannon, McMurry, Kim, Gleasner, Cheryl D., Lo, Chien-Chi, Li, Po-E, Vuyisich, Momchilo, Chain, Patrick S., Junier, Pilar

Anoxybacillus geothermalis strain GSsed3 is an endospore-forming thermophilic bacterium isolated from filter deposits in a geothermal site. This novel species has a larger genome size (7.2 Mb) than that of any other Anoxybacillus species, and it possesses genes that support its phenotypic metabolic characterization and suggest an intriguing link to metals.

, Filippidou, Sevasti, Wunderlin, Tina, Junier, Thomas, Jeanneret, Nicole, Johnson, Shannon, McMurry, Kim, Gleasner, Cheryl D, Lo, Chien-Chi, Li, Po-E, Vuyisich, Momchilo, Chain, Patrick S, Junier, Pilar

Bacillus alveayuensis strain 24KAM51 was isolated from a marine hydrothermal vent in Milos, Greece. Its genome depicts inter-esting features of halotolerance and resistance to heavy metals.