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Wunderlin, Tina
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Wunderlin, Tina
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Voici les éléments 1 - 10 sur 11
- PublicationMétadonnées seulementGenome Sequence of Bacillus alveayuensis Strain 24KAM51, a Halotolerant Thermophile Isolated from a Hydrothermal Vent(2015)
; ; ; - PublicationMétadonnées seulementEndospore-forming bacteria as new proxies to assess impact of eutrophication in Lake Geneva (Switzerland?France)(2014)
; ; - PublicationMétadonnées seulementPhysical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells(2016)
; ; ; Endospore formation is a survival strategy found among some bacteria from the phylum Firmicutes. During endospore formation, these bacteria enter a morpho-physiological resting state that enhances survival under adverse environmental conditions. Even though endospore-forming Firmicutes are one of the most frequently enriched and isolated bacterial groups in culturing studies, they are often absent from diversity studies based on molecular methods. The resistance of the spore core is considered one of the factors limiting the recovery of DNA from endospores. We developed a method that takes advantage of the higher resistance of endospores to separate them from other cells in a complex microbial community using physical, enzymatic and chemical lysis methods. The endospore-only preparation thus obtained can be used for re-culturing or to perform downstream analysis such as tailored DNA extraction optimized for endospores and subsequent DNA sequencing. This method, applied to sediment samples, has allowed the enrichment of endospores and after sequencing, has revealed a large diversity of endospore-formers in freshwater lake sediments. We expect that the application of this method to other samples will yield a similar outcome. - PublicationMétadonnées seulementGenome Sequence of Anoxybacillus geothermalis Strain GSsed3, a Novel Thermophilic Endospore-Forming Species(2015)
; ; ; ; - PublicationMétadonnées seulementGenome Sequence of Aeribacillus pallidus Strain GS3372, an Endospore-Forming Bacterium Isolated in a Deep Geothermal Reservoir(2015)
; ; ; - PublicationMétadonnées seulementQuantification of endospore-forming Firmicutes by quantitative PCR with the functional gene spo0A(2013)
; ; ; ; - PublicationMétadonnées seulementBacterial communities in trace metal contaminated lake sediments are dominated by endospore-forming bacteria(2014)
; ; ; - PublicationMétadonnées seulementUnder-detection of endospore-forming Firmicutes in metagenomic data(2015)
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- PublicationMétadonnées seulement