Isolation and characterization of polymorphic microsatellite loci in <i>Acanthoscelides obvelatus</i> Bridwell (Coleoptera: Bruchidae)

Five microsatellite loci were isolated from the bruchid <i>Acanthoscelides obvelatus</i> Bridwell (Coleoptera: Bruchidae). Each locus was polymorphic, with the number of alleles ranging from two to 15. We found high levels of within-population variation at most loci, with heterozygosity ranging from 0.182 to 0.900. Cross-species amplification of these loci was tested in two other species of the genus <i>Acanthoscelides</i>, <i>A. obtectus</i> Say and <i>A. argillaceus</i> Sharp.