Bafoudiosbulbins A, and B, two anti-salmonellal clerodane diterpenoids from <i>Dioscorea bulbifera</i> L. var <i>sativa</i>

Two clerodane diterpenoids, Bafoudiosbulbins A <b>1</b>, and B <b>2</b>, together with five known compounds: tetracosanoic acid, 1-(tetracosanoyl)-glycerol, trans-tetracosanylferulate, β-sitosterol and 3-O-β-d-glucopyranosyl-β-sitosterol were isolated from the tubers of <i>Dioscorea bulbifera</i> L. <i>var sativa</i>. Their structures were established by spectroscopic methods (1D and 2D-NMR, MS) and X-ray crystallographic diffraction analysis of compound <b>1</b>. The CH2Cl2-soluble portion of the crude extract and the two clerodanes were screened for anti-bacterial activity using both agar diffusion and broth dilution techniques against <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Staphylococcus aureus</i>, <i>Salmonella typhi</i>, <i>Salmonella paratyphi</i> A and <i>Salmonella paratyphi</i> B. They both showed significant activities against <i>P. aeruginosa</i>, <i>S. typhi</i>, <i>S. paratyphi</i> A and <i>S. paratyphi</i> B.