Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato
Author(s)
Rodriguez, Islay
Lienhard, Reto
Veuve, Marie Colette
Jouda, Fatima
Siegrist, Hans H
Fernandez, Carmen
Rodriguez, José Enrique
Date issued
2007
In
Memorias Do Instituto Oswaldo Cruz
Vol
8
No
102
From page
999
To page
1002
Subjects
Borrelia new medium BSK-H medium proteins BSK-II MEDIUM LYME BORRELIOSIS SWITZERLAND PREVALENCE ANTIBODIES DIVERSITY DISEASE TICKS MKP
Abstract
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barb our-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.
Publication type
journal article