In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system
Author(s)
Date issued
2009
In
Journal of Experimental Botany
Vol
1
No
60
From page
257
To page
267
Subjects
Heterodimerization in vivo protein-protein interaction protoplast split-ubiquitin Toc GTPases MEMBRANE-PROTEIN INTERACTIONS OUTER CHLOROPLAST MEMBRANE IMPORT MACHINERY SACCHAROMYCES-CEREVISIAE TOC-GTPASES ARABIDOPSIS PROTOPLASTS TRANSCRIPTION FACTORS SUCROSE TRANSPORTERS PREPROTEIN RECEPTOR PEROXISOME
Abstract
The GTPases atToc33 and atToc159 are pre-protein receptor components of the translocon complex at the outer chloroplast membrane in Arabidopsis. Despite their participation in the same complex in vivo, evidence for their interaction is still lacking. Here, a split-ubiquitin system is engineered for use in plants, and the in vivo interaction of the Toc GTPases in Arabidopsis and tobacco protoplasts is shown. Using the same method, the self-interaction of the peroxisomal membrane protein atPex11e is demonstrated. The finding suggests a more general suitability of the split-ubiquitin system as a plant in vivo interaction assay.
Publication type
journal article