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Photoactive sawhorse-type diruthenium tetracarbonyl complexes
Auteur(s)
Johnpeter, Justin P.
Schmitt, Frederic
Denoyelle-Di-Muro, Emmanuel
Wagnieres, Georges
Juillerat-Jeanneret, Lucienne
Date de parution
2012
In
Inorg. Chim. Acta
No
393
De la page
246
A la page
251
Résumé
Sawhorse-type diruthenium tetracarbonyl complexes incorporating carboxyphenyl porphyrin bridges and pyridine axial ligands were prepd., characterized and evaluated for use in photodynamic therapy (PDT) as potential photosensitizing and chemotherapeutic agents in several human cancer cells (A2780, A549, Me300, HeLa). The mono carboxyphenyl porphyrin derivs., 5-(4-carboxyphenyl)-10,15,20-triphenyl-21,23H-porphyrin (HOOCR1-H2) and 5-(4-carboxyphenyl)-10,15,20-triphenylporphyrin-Zn (HOOCR1-Zn), after reaction with Ru3(CO)12 and pyridine, give dinuclear [Ru2(CO)4(OOCR1-H2)2(NC5H5)2] (1) and [Ru2(CO)4(OOCR1-Zn)2(NC5H5)2] (2), resp. Under the same reaction conditions, the dicarboxyphenyl porphyrin derivs., 5,10-di(4-carboxyphenyl)-15,20-diphenyl-21,23H-porphyrin (HOOCR2-H2COOH) and 5,10-di(4-carboxyphenyl)-15,20-diphenylporphyrin-Zn (HOOCR2-ZnCOOH), give rise to the tetranuclear complexes, [{Ru2(CO)4(NC5H5)2}2(OOCR2-H2COO)2] (3) and [{Ru2(CO)4(NC5H5)2}2(OOCR2-ZnCOO)2] (4), in which two sawhorse diruthenium tetracarbonyl units are linked by the dicarboxyphenyl porphyrin ligands. When tested in human cancer cell lines, both Zn(II) metallo-porphyrin derivs. 2 and 4 and the tetranuclear deriv. 3 show some degree of cytotoxicity in the dark, but seem to present no phototoxicity upon irradn. at 652 nm. These results demonstrate the effect of the Zn(II) ion insertion into the porphyrin core, resulting in increased cytotoxicity and decreased phototoxicity. However, complex 1, the less cytotoxic deriv. with IC50 > 170 ?M in HeLa cervix and A2780 ovarian cancer cell lines, shows an excellent phototoxicity toward these cancer cell lines with LD50 comprised between 4.5 and 7.5 J/cm2 (irradiance 30 mW/cm2) at 5 ?M concn. (incubation time: 24 h). Overall, an excellent ratio between photo- and cytotoxicity was found for the metal-free porphyrin deriv. [Ru2(CO)4(OOCR1-H2)2(NC5H5)2] (1). [on SciFinder(R)]
Identifiants
Type de publication
journal article
