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  4. Real-time Amyloid Aggregation Monitoring with a Photonic Crystal-based Approach
 
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Real-time Amyloid Aggregation Monitoring with a Photonic Crystal-based Approach

Auteur(s)
Santi, Sara 
Institut de chimie 
Musi, Valeria
Descrovi, Emiliano
Paeder, Vincent
Di Francesco, Joab 
Institut de physique 
Hvozdara, Lubos
van der Wal, Peter
Lashuel, Hilal A.
Pastore, Annalisa
Neier, Reinhard 
Institut de chimie 
Herzig, Hans Peter
Date de parution
2013
In
ChemPhysChem
Vol.
15
No
14
De la page
3476
A la page
3482
Mots-clés
  • Optical sensors (biosensors
  • refractive index
  • real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Surface wave (bloch
  • real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Organelle (fibril
  • real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Adsorption (interfacial
  • real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Polymerization (oligomerization
  • real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Biosensors (optical
  • refractive index
  • real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Aggregation
  • Photonic crystals
  • Refractive index
  • Refractometers
  • Self-assembled monolayers
  • Simulation and Modeling
  • Surface treatment (real-time amyloid aggregation monitoring with photonic crystal-based approach)
  • Polyoxyalkylenes Role: ARU (Analytical role
  • unclassified)
  • BUU (Biological use
  • unclassified)
  • PEP (Physical
  • engineering or chemical process)
  • TEM (Technical or engineered material use)
  • ANST (Analytical study)
  • BIOL (Biological study)
  • PROC (Process)
  • amyloid aggregation biosensor refractometry photonic crystal
  • Optical sensors (bios...

  • refractive index

  • real-time amyloid agg...

  • Surface wave (bloch

  • real-time amyloid agg...

  • Organelle (fibril

  • real-time amyloid agg...

  • Adsorption (interfaci...

  • real-time amyloid agg...

  • Polymerization (oligo...

  • real-time amyloid agg...

  • Biosensors (optical

  • refractive index

  • real-time amyloid agg...

  • Aggregation

  • Photonic crystals

  • Refractive index

  • Refractometers

  • Self-assembled monola...

  • Simulation and Modeli...

  • Surface treatment (re...

  • Polyoxyalkylenes Role...

  • unclassified)

  • BUU (Biological use

  • unclassified)

  • PEP (Physical

  • engineering or chemic...

  • TEM (Technical or eng...

  • ANST (Analytical stud...

  • BIOL (Biological stud...

  • PROC (Process)

  • amyloid aggregation b...

Résumé
We propose the application of a new label-free optical technique based on photonic nanostructures to real-time monitor the amyloid-beta 1-42 (A?(1-42)) fibrillization, including the early stages of the aggregation process, which are related to the onset of the Alzheimer's Disease (AD). The aggregation of A? peptides into amyloid fibrils has commonly been assocd. with neuronal death, which culminates in the clin. features of the incurable degenerative AD. Recent studies revealed that cell toxicity is detd. by the formation of sol. oligomeric forms of A? peptides in the early stages of aggregation. At this phase, classical amyloid detection techniques lack in sensitivity. Upon a chem. passivation of the sensing surface by means of polyethylene glycol, the proposed approach allows an accurate, real-time monitoring of the refractive index variation of the soln., wherein A?(1-42) peptides are aggregating. This measurement is directly related to the aggregation state of the peptide throughout oligomerization and subsequent fibrillization. Our findings open new perspectives in the understanding of the dynamics of amyloid formation, and validate this approach as a new and powerful method to screen aggregation at early stages. [on SciFinder(R)]
Identifiants
https://libra.unine.ch/handle/123456789/21450
Type de publication
journal article
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