A Cyclometallated Platinum Complex as a Selective Optical Switch for Quadruplex DNA
Author(s)
Suntharalingam, Kogularamanan
Leczkowska, Anna
Furrer, Mona A.
Wu, Yilei
Kuimova, Marina K.
White, Andrew J. P.
Vilar, Ramon
Date issued
2012
In
Chem. - Eur. J.
Vol
51
No
18
From page
16277
To page
16282
Subjects
cyclometallated platinum complex prepn quadruplex DNA nucleolus fluorescence microscopy host guest cyclometallated platinum complex quadruplex DNA imaging
Abstract
Herein the authors have presented a new cyclometallated platinum(II) complex the emission of which is switch-ed-on in the presence of DNA. The complex interacts with a 1000-fold selectivity with quadruplex DNA over duplex DNA. More interestingly, its emission is switched-on (ca. 35-fold) in the presence of c-Myc gene and 6183 NT quadruplex DNA, but no significant change in intensity was obsd. upon addn. of the same amts. of duplex ds26 DNA (and only a slight change upon addn. of ct-DNA). Upon encapsulation of this complex inside a hexaruthenium cage, the authors have been able to deliver the complex inside living cells, allowing to carry out confocal fluorescence microscopy studies. These studies have shown that the compd. is released from the cage once inside the cell and a considerable amt. of it penetrates the nucleus and localizes in the nucleoli. [on SciFinder(R)]
Publication type
journal article