DNA extraction from Ascaris suum muscle tissue
Author(s)
Di Mito, Carmela
Date issued
1998
In
Parasitology Research
Vol
7
No
84
From page
596
To page
597
Abstract
A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase It allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
Publication type
journal article