Variations of adenyl nucleotide content in two potato cultivars stored at 15	°C. I. Method for the determination of ATP level and first results

Siegenthaler, Paul-André; Biotto, C

doi:10.1007/BF02358716

Variations of adenyl nucleotide content in two potato cultivars stored at 15 °C. I. Method for the determination of ATP level and first results

Author(s)

Siegenthaler, Paul-André

Biotto, C

Date issued

1994

In

Potato Research, Springer

Vol

37

From page

151

To page

160

Subjects

Aging apyrase ATP bioluminescence bioenergetic metabolism luciferase luciferin <i>Solanum tuberosum</i> L.

Abstract

A method for determining ATP content based on sulfuric acid extraction and luciferin-luciferase bioluminescence assay was developed for potato tubers. The inactivation of apyrase by a thermal treatment as well as the ATP stability in acidic potato extracts stored at -25°C have been particularly considered. The proposed method is simple, reliable, sensitive and can be used for automatic purposes. This method has been applied to the study of the variation of ATP levels in stored potato tubers (cvs Ukama and Granola). The large changes occurring over a 9 month period are discussed in correlation with dormancy, sprouting and tuberization.